Rat anti-Mouse CD31 Monoclonal Antibody | anti-CD31 antibody
Rat Anti Mouse CD31: FITC
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Application Data
(Published Customer Image:Mouse CD31 antibody, clone ER-MP12 used for the demonstration of vasculature in mouse brain by immunofluorescence.Image caption:Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n?=?5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean?+/-SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p?)
Application Data
(Rat anti Mouse CD31 antibody, clone ER-MP12 used for the detection of blood vessels in an experimental murine tumor model by immunohistofluorescence.Image caption:Percentage of pixels positive for NG2 in 4T1 (n = 5) and RM11 (n = 4 and n = 6) tumors (A,D) from WT and beta3-KO mice were calculated using immunofluorescent images. No statistical differences in 4T1 (p = 0.90) or RM11 (p = 0.23) were found. Mean +/- SD. Representative images of NG2-staining from both genotypes of 4T1 (B,C) (NG2 green, CD31 red) and RM11 (E,F) (NG2 red) tumors are shown. Scale bars indicate 50 mum.From: Reigstad I, Sortland K, Skogstrand T, Reed RK, Stuhr L.The Effect of Stromal Integrin beta3-Deficiency on Two Different Tumors in Mice.Cancers (Basel). 2016 Jan 12;8(1). pii: E14.)
Application Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Application Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Application Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Application Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and Pacific Blue conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
Application Data
(Figure A. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine peripheral blood in the presence of murine SeroBlock (BUF041A).)
Application Data
(Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)
CD31 is also expressed on a major population of macrophage/dendritic cell precursors in the bone marrow. Studies show that clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).
