Application Data
(Published customer image: Dynamics of Th17 cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+ROR?+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 um.Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)
Mouse CD4 Monoclonal Antibody | anti-CD4 antibody
MOUSE ANTI RAT CD4 (DOMAIN 1)
Purified IgG - liquid
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Immunohistology - Paraffin: Application Note: PLP fixation is recommended for optimal results, see Whiteland et al. for details
Preparation
Fusion Partners
Shelf Life: 18 months from date of despatch.
Application Data
(Published customer image: Dynamics of Th17 cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+ROR?+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 um.Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)
Application Data
(Published customer image: Dynamics of Th17 cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+ROR?+ cell (arrow) observed around blood vessels (BV). Bar scale = 30 um.Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)
Application Data
(Staining of frozen rat spleen with Mouse anti Rat CD4)
Application Data
(Staining of frozen rat spleen with Mouse anti Rat CD4)
Application Data
(Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. High power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. High power)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD4)
Application Data
(Staining of rat splenocytes with Mouse anti Rat CD4)
Application Data
(Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. Low power)
Application Data
(Immunoperoxidase staining of rat lymph node cryosetion using Mouse anti Rat CD4 antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 for detection. Low power)
Application Data
(Staining of stimulated rat spleen cells with Mouse anti Rat CD4)
Application Data
(Staining of stimulated rat spleen cells with Mouse anti Rat CD4)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)
Application Data
(Published customer image: Dynamics of T-regulatory cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+Foxp3+ cell (arrows) found around blood vessels (BV). Bar scale = 30 um.From: Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)
Application Data
(Published customer image: Dynamics of T-regulatory cells. D -F) Photographs of double immunolabelled sections showing a representative CD4+Foxp3+ cell (arrows) found around blood vessels (BV). Bar scale = 30 um.From: Almolda B, Costa M, Montoya M, Gonz¡lez B, Castellano B (2011) Increase in Th17 and T-reg Lymphocytes and Decrease of IL22 Correlate with the Recovery Phase of Acute EAE IN Rat. PLoS ONE 6(11): e27473.)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)
Application Data
(Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)
Application Data
(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)
Application Data
(Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD169, clone ED3 , red in A and Mouse anti Rat CD4, clone W3/25 , green in B. C is the merged image with nuclei counter-staind blue using DAPI. High power)
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Product Notes
The CD4 cd4 (Catalog #AAA11995) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunohistology Paraffin*. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200 Immunohistology - Paraffin: Application Note: PLP fixation is recommended for optimal results, see Whiteland et al. for details. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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