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Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4)
Rat CD4 Monoclonal Antibody | anti-CD4 antibody
RAT ANTI MOUSE CD4
Gene Names
Cd4; L3T4; Ly-4
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD4, Antibody; RAT ANTI MOUSE CD4; anti-CD4 antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
YTS191.1
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1 mg/ml (varies by lot)
Sequence Length
217
Applicable Applications for anti-CD4 antibody
Immunohistology Frozen*, Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4)
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405)
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4)
Application Data
(Staining of mouse spleen with Rat anti Mouse CD4)
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4: APC)
Application Data
(Staining of mouse spleen cells with Rat anti Mouse CD4: APC)
Related Product Information for anti-CD4 antibody
Rat anti Mouse CD4 antibody, clone YTS191.1 recognizes the murine CD4 cell surface antigen, expressed by a subset of T lymphocytes. Rat anti Mouse CD4 antibody, clone YTS191.1 exhibits depleting activity when used in vivo (Bemelman et al. 1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,473 Da
NCBI Official Full Name
T-cell surface glycoprotein CD4
NCBI Official Synonym Full Names
CD4 antigen
NCBI Official Symbol
Cd4
NCBI Official Synonym Symbols
L3T4; Ly-4
NCBI Protein Information
T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
UniProt Protein Name
T-cell surface glycoprotein CD4
UniProt Gene Name
Cd4
UniProt Entry Name
CD4_MOUSE
Similar Products
Product Notes
The CD4 cd4 (Catalog #AAA11939) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD4 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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