Rat CD4 Monoclonal Antibody | anti-CD4 antibody

RAT ANTI MOUSE CD4:Low Endotoxin

Cat. Num. AAA11900

• Gene Names: Cd4; L3T4; Ly-4
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Functional Assay
• Synonyms: CD4, Antibody; RAT ANTI MOUSE CD4:Low Endotoxin; anti-CD4 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: YTS191.1
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 217

• Applicable Applications for anti-CD4 antibody: Immunohistology Frozen*, Flow cytometry (FC/FACS), Functional Assays (FN)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD4)

Application Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)

Application Data

(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405)

Application Data

(Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)

Application Data

(Staining of mouse spleen with Rat anti Mouse CD4)

Application Data

(Staining of mouse spleen cells with Rat anti Mouse CD4: APC)

Related Product Information for anti-CD4 antibody

Rat anti Mouse CD4 antibody, clone YTS191.1 recognizes the murine CD4 cell surface antigen, expressed by a subset of T lymphocytes. Rat anti Mouse CD4 antibody, clone YTS191.1 exhibits depleting activity when used in vivo (Bemelman et al. 1998).

NCBI and Uniprot Product Information

• NCBI GI #: 7304953
• NCBI GeneID: 12504
• NCBI Accession #: NP_038516.1
• NCBI GenBank Nucleotide #: NM_013488.2
• UniProt Accession #: P06332
• Molecular Weight: 24,473 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 antigen
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: L3T4; Ly-4
• NCBI Protein Information: T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)
• UniProt Protein Name: T-cell surface glycoprotein CD4
• UniProt Gene Name: Cd4
• UniProt Entry Name: CD4_MOUSE