Mouse CD44 Monoclonal Antibody | anti-CD44 antibody

MOUSE ANTI HUMAN CD44:FITC

Cat. Num. AAA11893

• Gene Names: CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Purity: Purified
• Synonyms: CD44; Monoclonal Antibody; MOUSE ANTI HUMAN CD44:FITC; LHR; MDU2; MDU3; MIC4; anti-CD44 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: F10-44-2
• Specificity: CD44
• Purity/Purification: Purified
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)

• Applicable Applications for anti-CD44 antibody: Flow cytometry (FC/FACS); Immunohistochemistry - Frozen/Paraffin (IHC-F/P); Immunofluorescence (IF).
• Application Notes: Flow Cytometry:: 1/10 - 1/20; Immunohistology - Paraffin: 1/50 - 1/100; ; Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.
• Target Species: Human
• Species Cross Reactivity: Reacts with: Cynomolgus monkey; N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Buffer: Solution Phosphate buffered saline
• Preservative Stabilisers: 0.09% Sodium Azide
• Carrier Free: Yes
• Approx. Protein Concentrations: IgG concentration 1mg/ml
• Immunogen: Human T lymphocytes.
• Fusion Partners: Spleen cells of immunised BALB/c mice were fused with cells from the mouse NS1 myeloma line.
• Histology Positive: Human Tonsil
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of despatch.

Application Data

(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody followed by HRP-polymer detection and DAB substrate development (high power) following antigen retrieval using citrate buffer at pH6.2)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:FITC)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:Azide free)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:RPE)

Application Data

(Staining of human peripheral blood granulocytes with Mouse anti Human CD44:Alexa Fluor 647)

Application Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD44:Biotin)

Application Data

(Formalin fixed, paraffin embedded human breast cancer biopsy stained with Mouse anti Human CD44 antibody followed by HRP-polymer detection and DAB substrate development (low power) following antigen retrieval using citrate buffer at pH6.2)

Related Product Information for anti-CD44 antibody

Mouse anti Human CD44 antibody, clone F10-44-2 recognizes human CD44, also known as Epican, HCAM, Phagocytic Glycoprotein 1 (PGP-1) or Hyaluronate receptor. Human CD44 is a single pass, type I transmembrane glycoprotein of variable molecular weight ranging from ~90kDa to ~220kDa depending on alternate splicing of the variable region exons and on the degree of glycosylation. CD44 is expressed on multiple cell types and is involved in multiple functions including cell-cell interactions and cell-extracellular matrix binding. Hyaluronan, a high molecular weight polysaccharide component of the extracellular matrix acts as the principal ligand for the CD44 receptor (Laurent and Fraser 1992).

CD44 isoforms containing one or more sequences encoded by the variant region exons have a much more restricted expression pattern both in terms of organ specificity and immune activation (Mackay et al. 1994).

Mouse anti Human CD44 antibody, clone F10-44-2 recognizes an epitope on human CD44 outside the regions coded for by the variable region exons and is thus expected to recognize all isoforms of human CD44 (Mackay et al. 1994).

NCBI and Uniprot Product Information

• NCBI GI #: 48255935
• NCBI GeneID: 960
• NCBI Accession #: NP_000601.3
• NCBI GenBank Nucleotide #: NM_000610.3
• UniProt Accession #: P16070; O95370; P22511; Q04858; Q13419; Q13957; Q13958; Q13959; A5YRN9; B6EAT9; D3DR12; D3DR13
• NCBI Official Full Name: CD44 antigen isoform 1
• NCBI Official Synonym Full Names: CD44 molecule (Indian blood group)
• NCBI Official Symbol: CD44
• NCBI Official Synonym Symbols: IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
• NCBI Protein Information: CD44 antigen; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; epican; extracellular matrix receptor III; hematopoietic cell E- and L-selectin ligand; heparan sulfate proteoglycan; homing function and Indian blood group system; hyaluronate receptor; phagocytic glycoprotein 1
• UniProt Protein Name: CD44 antigen
• UniProt Gene Name: CD44
• UniProt Synonym Gene Names: LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I
• UniProt Entry Name: CD44_HUMAN