FCM (Flow Cytometry)
Rat CD45 Monoclonal Antibody | anti-CD45 antibody
Anti-Mouse CD45, Purified (Clone IBL-5/25) (rat IgG1)
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
Purified from ascitic fluid via Protein G Chromatography
Synonyms
CD45, Antibody; Anti-Mouse CD45, Purified (Clone IBL-5/25) (rat IgG1); CD45, Purified (Clone IBL-5/25) (rat IgG1); Purified Anti-Mouse CD45 Monoclonal Antibody; anti-CD45 antibody
Host
Rat
Clonality
Monoclonal
Isotype
Rat IgG1
Clone Number
IBL-5/25
Specificity
Mouse CD45 (L-CA)
Purity/Purification
Purified from ascitic fluid via Protein G Chromatography
Form/Format
Purified IgG buffered in PBS and 0.02% NaN3. For maximum recovery of contents, spin down tube before use.
Applicable Applications for anti-CD45 antibody
Immunohistochemistry (IHC) (acetone-fixed frozen sections), Flow Cytometry (FC), and Immunoprecipitation (IP)
Application Notes
N.B. Appropriate control samples should always be included in any labeling studies.
*For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
*For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use.
Hybridoma Production
Immunization:
Immunogen: IL-3 dependent mast cells derived from WB- +/+ mice; Donor: Wistar spleen
Fusion Partner: X63.653. Ag8
Immunogen: IL-3 dependent mast cells derived from WB- +/+ mice; Donor: Wistar spleen
Fusion Partner: X63.653. Ag8
Test Results
Mouse Strain: BALB/c
Cell Concentration: 1x106 cells per tests
Antibody Concentration Used: 0.1 ug/106 cells,
Cell Source: Mesenteric Lymph Node; Percentage of cells stained above control: 99.8%
Cell Concentration: 1x106 cells per tests
Antibody Concentration Used: 0.1 ug/106 cells,
Cell Source: Mesenteric Lymph Node; Percentage of cells stained above control: 99.8%
Preparation and Storage
For all formats, store at 4°C. For long term storage (Purified), aliquot and freeze unused portion at -20°C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
FCM (Flow Cytometry)
FCM (Flow Cytometry)
Related Product Information for anti-CD45 antibody
Anti-mouse CD45 monoclonal antibody detects CD45 (L-CA) which is a transmembrane phosphotyrosine phosphatase expressed on leukocytes. This mAb induces the in vitro clustering of mouse lymphocytes (both T and B cells).
Applications for this clone include immunohistochemistry (acetone-fixed frozen sections), flow cytometry and immunoprecipitation.
Applications for this clone include immunohistochemistry (acetone-fixed frozen sections), flow cytometry and immunoprecipitation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD45
NCBI Official Symbol
ptprc
NCBI Protein Information
CD45
Similar Products
Product Notes
The CD45 (Catalog #AAA14270) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (acetone-fixed frozen sections), Flow Cytometry (FC), and Immunoprecipitation (IP). N.B. Appropriate control samples should always be included in any labeling studies. *For optimal results in various applications, it is recommended that each investigator determine dilutions appropriate for individual use. Researchers should empirically determine the suitability of the CD45 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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