WB (Western Blot)
(Detection of human CD45 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
CD45 recombinant antibody
Rabbit anti-CD45 Recombinant Monoclonal Antibody [BL-178-12C7]
IP: 20 ul/mg lysate
IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells.
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CD45 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human CD45 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, MOLT-4, HeLa, and SR cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 3) used for IP at 20 ul/mg lysate. CD45 was also immunoprecipitated by a previous lot of this antibody (AAA23790 lot 2). For blotting immunoprecipitated CD45, AAA23790 was used at 1:1000. Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human CD45 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 3) used for IP at 20 ul/mg lysate. CD45 was also immunoprecipitated by a previous lot of this antibody (AAA23790 lot 2). For blotting immunoprecipitated CD45, AAA23790 was used at 1:1000. Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohistochemistry)
(Detection of human CD45 (red) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry)
(Detection of human CD45 (red) by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistchemistry)
(Detection of human CD45 by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-rabbit IgG .)
IHC (Immunohistchemistry)
(Detection of human CD45 by immunhistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human CD45 by immunohistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human CD45 by immunohistochemistry. Sample: FFPE section of appendix. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human CD45 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry)
(Detection of human CD45 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Jurkat cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry)
(Detection of human CD45 by immunocytochemistry. Sample: FFPE section of Daudi cells. Antibody: Rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human CD45 by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody:1E6 cells were incubated with 1 ul of rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry)
(Detection of human CD45 by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody:1E6 cells were incubated with 1 ul of rabbit anti-CD45 recombinant monoclonal antibody [BL-178-12C7] (AAA23790 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
NCBI and Uniprot Product Information
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Product Notes
The CD45 ptprc (Catalog #AAA23790) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CD45 Recombinant Monoclonal Antibody [BL-178-12C7] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry-Immunofluorescence (IHC-IF), Flow Cytometry (FC/FACS). WB: 1:1000 IP: 20 ul/mg lysate IHC: 1:100-1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ICC: 1:100-1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. IHC-IF: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. FC/FACS: Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 ul per 1 x 10^6 cells. Researchers should empirically determine the suitability of the CD45 ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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