SDS-PAGE
(The antibody wsas purified by protein G affinity achromatography from cell culture supernatants and verified by SDS-Page (Fig.2).Fig.2: SDS-PAGE analysis of purified YD-4E9 monoclonal antibody.Lane 1: molecular weight markerLane 2: 2ug of purified YD-4E9 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Rat anti-Human CLAUDIN9 Monoclonal Antibody | anti-CLDN9 antibody
anti-human Claudin 9 monoclonal antibody
Reactivity
Human
Applications
Flow Cytometry, ELISA, ELISA
Purity
Protein G
Synonyms
CLAUDIN9, Antibody; anti-human Claudin 9 monoclonal antibody; CLDN9; anti-CLDN9 antibody
Host
Rat
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
YD-4E9
Specificity
anti-human Claudin 9
Purity/Purification
Protein G
Form/Format
phosphate buffered saline, pH 7.2
Sequence Length
217
Applicable Applications for anti-CLDN9 antibody
Flow Cytometry (FC), CELISA, ELISA (EIA)
Application Notes
ELISA: 1:200 - 1:400
Flow Cytometry: 1.2 ug/106 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Flow Cytometry: 1.2 ug/106 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
Immunogen
genetic immunization with cDNA encoding human Claudin 9
Selection
based on recognition of the complete native protein expressed on transfected mammalian cells
Preparation and Storage
Short term: 2°C - 8°C; long term: -20°C (avoid repeated freezing and thawing)
SDS-PAGE
(The antibody wsas purified by protein G affinity achromatography from cell culture supernatants and verified by SDS-Page (Fig.2).Fig.2: SDS-PAGE analysis of purified YD-4E9 monoclonal antibody.Lane 1: molecular weight markerLane 2: 2ug of purified YD-4E9 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
SDS-PAGE
(The antibody wsas purified by protein G affinity achromatography from cell culture supernatants and verified by SDS-Page (Fig.2).Fig.2: SDS-PAGE analysis of purified YD-4E9 monoclonal antibody.Lane 1: molecular weight markerLane 2: 2ug of purified YD-4E9 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
FCM (Flow Cytometry)
(Fig.1: AAA14098. BOSC23 cells were transiently transfected with an expression vector encoding either Claudin 9 (red curve) or an irrelevant protein (control transfectant). Binding of YD-4E9 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with Claudin 9 transfected cells.)
FCM (Flow Cytometry)
(Fig.1: AAA14098. BOSC23 cells were transiently transfected with an expression vector encoding either Claudin 9 (red curve) or an irrelevant protein (control transfectant). Binding of YD-4E9 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with Claudin 9 transfected cells.)
Related Product Information for anti-CLDN9 antibody
Description: Purified monoclonal rat antibody
Backgroud: Claudin 9 belongs to the claudin family which constitutes a large group of four-transmembrane domain proteins (1). Claudins are integral tight junction proteins that are responsible for maintaining the integrity of epithelial cell architecture, the control of paracellular transport and cell polarity. The expression pattern of claudins is tissue specific, most tissues express multiple claudins, which can interact in both homotypic and heterotypic fashion to form the tight junction strands (2). Several claudin proteins have been shown to be abnormally expressed in cancers. The differential expression of these proteins between tumour and normal cells, in addition to their membrane localisation, makes them prime candidates for cancer therapy (2). Members of the claudin family, including Claudin 6 and 9 are expressed in the liver and play a critical role in Hepatitis C Infection. They function as coreceptors in HCV entry and assume a role in HCV dissemination, replication and pathogenesis (3, 4).
Backgroud: Claudin 9 belongs to the claudin family which constitutes a large group of four-transmembrane domain proteins (1). Claudins are integral tight junction proteins that are responsible for maintaining the integrity of epithelial cell architecture, the control of paracellular transport and cell polarity. The expression pattern of claudins is tissue specific, most tissues express multiple claudins, which can interact in both homotypic and heterotypic fashion to form the tight junction strands (2). Several claudin proteins have been shown to be abnormally expressed in cancers. The differential expression of these proteins between tumour and normal cells, in addition to their membrane localisation, makes them prime candidates for cancer therapy (2). Members of the claudin family, including Claudin 6 and 9 are expressed in the liver and play a critical role in Hepatitis C Infection. They function as coreceptors in HCV entry and assume a role in HCV dissemination, replication and pathogenesis (3, 4).
References
Kazumasa M, Mikio F, Kazushi F, Shoichiro T (1999). Claudin multigene family encoding fourtransmembrane
domain protein components of tight junction strands. Proc Natl Acad Sci U S A.
19; 96(2): 511–516.
Morin PJ (2005). Claudin proteins in human cancer: promising new targets for diagnosis and therapy. Cancer Res. 1;65(21):9603-6.
Zheng A, Yuan F, Li Y, Zhu F, Hou P, Li J, Song X, Ding M, Deng H (2007). Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus. J Virol. 81(22):12465-71.
Fofana I, Zona L, Thumann C, Heydmann L, Durand SC, Lupberger J, Blum HE, Pessaux P, Gondeau C, Reynolds GM, McKeating JA, Grunert F, Thompson J, Zeisel MB, Baumert TF (2013). Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies. J Virol. 87(18):10405-10
Morin PJ (2005). Claudin proteins in human cancer: promising new targets for diagnosis and therapy. Cancer Res. 1;65(21):9603-6.
Zheng A, Yuan F, Li Y, Zhu F, Hou P, Li J, Song X, Ding M, Deng H (2007). Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus. J Virol. 81(22):12465-71.
Fofana I, Zona L, Thumann C, Heydmann L, Durand SC, Lupberger J, Blum HE, Pessaux P, Gondeau C, Reynolds GM, McKeating JA, Grunert F, Thompson J, Zeisel MB, Baumert TF (2013). Functional analysis of claudin-6 and claudin-9 as entry factors for hepatitis C virus infection of human hepatocytes by using monoclonal antibodies. J Virol. 87(18):10405-10
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
claudin-9
NCBI Official Synonym Full Names
claudin 9
NCBI Official Symbol
CLDN9
NCBI Protein Information
claudin-9
UniProt Gene Name
CLDN9
UniProt Entry Name
CLD9_HUMAN
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Product Notes
The CLDN9 cldn9 (Catalog #AAA14098) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The anti-human Claudin 9 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CLAUDIN9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), CELISA, ELISA (EIA). ELISA: 1:200 - 1:400 Flow Cytometry: 1.2 ug/106 cells CELISA: 1:200 - 1:400 For each application a titration should be performed to determine the optimal concentration. Researchers should empirically determine the suitability of the CLDN9 cldn9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLAUDIN9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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