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Rabbit Creatine Kinase B Type Monoclonal Antibody | anti-CKB antibody
Creatine Kinase B Type Antibody
Gene Names
CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Creatine Kinase B Type, Antibody; Creatine Kinase B Type Antibody; B CK antibody; B-CK antibody; BB-CK antibody; BCK antibody; Brain creatine kinase antibody; Ckb antibody; CKBB antibody; Creatine kinase B antibody; Creatine kinase B chain antibody; Creatine kinase B type antibody; Creatine kinase B-type antibody; Creatine Kinase BB Isoenzyme antibody; Creatine kinase brain antibody; Creatine kinase brain type antibody; Creatine phosphokinase BB antibody; Epididymis luminal protein 211 antibody; Epididymis secretory protein Li 29 antibody; HEL 211 antibody; HEL S 29 antibody; KCRB_HUMAN antibody; anti-CKB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB78-34
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CKB antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of 293T cells with Creatine kinase B type antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Creatine kinase B type in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Creatine kinase B type in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Creatine kinase B type antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Creatine kinase B type on different lysates using anti-Creatine kinase B type antibody at 1/500 dilution. Positive control: Lane 1: Mouse brain Lane 2: Mouse brain Lane 3: Rat brain Lane 4: 293)
Related Product Information for anti-CKB antibody
Creatine kinases (CKs) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. Creatine kinases provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems including muscle, electrocytes, retina photoreceptor cells, brain cells, kidney, salt glands, myometrium, placenta, pancreas, thymus, thyroid, intestinal epithelial cells, endothelial cells, cartilage and bone cells, macrophages, blood platelets, and tumor and cancer cells. Human cytoplasmic creatine kinase-B, also designated CK-B and BCK, is a 381 amino acid, brain tissue specific isoform of creatine kinase. Human cytoplasmic creatine kinase-muscle (CK-M, MCK) is a muscle tissue-specific isoform of creatine kinase. Human cytoplasmic creatine kinase-Mi (Mi-CK, MtCK) is a 416 amino acid mitochondrial-specific isoform of creatine kinase. Cytosolic creatine kinases are important in the energetic regulation of Ca2+-pumps and in the maintenance of Ca2+-homeostasis.
Product Categories/Family for anti-CKB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
381
NCBI Official Full Name
Creatine kinase B-type
NCBI Official Synonym Full Names
creatine kinase, brain
NCBI Official Symbol
CKB
NCBI Official Synonym Symbols
BCK; B-CK; CKBB; HEL-211; HEL-S-29
NCBI Protein Information
creatine kinase B-type; creatine kinase-B; creatine kinase B chain; epididymis luminal protein 211; epididymis secretory protein Li 29
UniProt Protein Name
Creatine kinase B-type
UniProt Gene Name
CKB
UniProt Synonym Gene Names
CKBB
UniProt Entry Name
KCRB_HUMAN
