Mouse anti-Chicken CSF1R Monoclonal Antibody | anti-CSF1R antibody

MOUSE ANTI CHICKEN CSF1R

Cat. Num. AAA12252

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• Reactivity: Chicken
• Applications: Immunohistochemistry, Flow Cytometry, Immunofluorescence
• Synonyms: CSF1R, Antibody; MOUSE ANTI CHICKEN CSF1R; anti-CSF1R antibody

• Host: Mouse
• Reactivity: Chicken
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: ROS-AV170
• Specificity: CSF1R
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 454

• Applicable Applications for anti-CSF1R antibody: Immunohistochemistry (IHC) Frozen, Flow Cytometry (FC), Immunofluorescence (IF)
• Application Notes: Flow Cytometry: 1/50; 1/200; Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul; Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
• Immunogen: Purified ChCSF1R-Fc fusion protein
• Fusion Partners: Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line
• Reactivity Notes: Species Cross Reactivity: Reacts with: Grey partridge, Guinea Fowl; Does not react with:Goose, Duck, Quail, Turkey, Mouse, Rat; N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information.
• Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
• Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Buffer Solution: Phosphate buffered saline
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Application Data

(Mouse anti Chicken CSF1R (CD115) immunohistochemical staining of guinea fowl, Agelastes meleagredisbursa of Fabricius.Clone ROS-AV170 specifically stains the bursal secretory dendritic cells (BSDC) located in the medulla. Clone ROS-AV170 also recognizes some CSF1R+ve macrophages in the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary)

Application Data

(Mouse anti Chicken CSF1R (CD115) immunohistochemical staining of quail, Cortunix cortunix, bursa of Fabricius.Cryostat sections were cut from 4 week old quail bursa of Fabricius. Clone ROS-AV170 specifically stains the bursal secretory dendritic cells (BSDC) located in the medulla and the macrophages of the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary)

FCM (Flow Cytometry)

(Staining of CSF1R transfected CHO cells with Mouse anti Chicken CSF1R followed by FITC conjugated Goat anti Mouse IgG following permeabilization with Leucoperm™ (BUF09))

FCM (Flow Cytometry)

(Staining of CSF1R transfected CHO cells with Mouse anti Chicken CSF1R following permeabilization with Leucoperm™ (BUF09))

FCM (Flow Cytometry)

(Staining of CSF1R transfected CHO cells with Mouse anti Chicken CSF1R following permeabilization with Leucoperm™ (BUF09))

Application Data

(Mouse anti Chicken CSF1R (CD115) immunohistochemical staining of Grey Patridge, Perdix perdix bursa of Fabricius.Cryostat sections we cut from 4 week old partridge bursa of Fabricius. Clone ROS-AV170 specifically recognized membrane expressed antigen on the bursal secretory dendritic cells (BSDC) in the medulla and by the macrophages (arrows) of the interfollicular connective tissue. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary)

Application Data

(Mouse anti Chicken CSF1R (CD115) immunofluorescence staining of chicken bursa of Fabricius. Cryostat sections of 6 week old chicken bursa of Fabricius. A) Clone ROS-AV170 identifies strong membrane-bound immunoreaction on the cell surface of the bursal secretory dendritic cells (BSDC). B) Double immunofluorescence staining with Mouse anti vimentin, clone 3B4 (green), (product code OBT3104) and Mouse anti Chicken CSF1R, clone ROS-AV170 (red), (product code This product) shows that colocalization of vimentin with CSF1R occurs only in bursal dendritic cells. Image courtesy of Dr Nandor Nagy, Semmelweis University, Hungary)

Related Product Information for anti-CSF1R antibody

Mouse anti Chicken CSF1R, clone ROS-AV170, recognizes the chicken homologue of human CSF1R, also known as CD115, CSF-1R, M-CSF receptor and c-fms. CSF1R is a single pass type 1 transmembrane protein belonging to the type III protein kinase family and is the cell surface receptor for both CSF1 and IL-34. Evidence from comparative sequence analysis across a range of vertebrate species indicates that CSF1 and IL-34 interact with distinct regions of CSF1R (Garceau V. et al. 2010). CSF1R cell surface expression appears to be one of the earliest events in macrophage lineage cells (Garceau V. et al. 2010). Clone ROS-AV170 is a monoclonal antibody that has been generated specifically to recognize chicken CSF1R. Clone ROS-AV170 has been shown to recognize the CSF1R antigen in some, but not all closely related avian species. Clone ROS-AV170 secretes an antibody that is an excellent marker for chicken macrophages.

NCBI and Uniprot Product Information

• NCBI GI #: 125858971
• NCBI GeneID: 1436
• NCBI Official Full Name: CSF1R protein, partial
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R
• UniProt Entry Name: CSF1R_HUMAN