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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

Mouse anti-Human Cystatin C Monoclonal Antibody | anti-CST3 antibody

Cystatin C Mouse Monoclonal Antibody (7F11)

Gene Names
CST3; ARMD11
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry, ELISA
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Synonyms
Cystatin C; Monoclonal Antibody; Cystatin C Mouse Monoclonal Antibody (7F11); CST3; anti-CST3 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Clone Number
7F11
Specificity
Cystatin C protein detects endogenous levels of CST3
Purity/Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CST3 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA)
Application Notes
IF: 1:50-200
WB: 1:1000-2000
IHC: 1:100-200
Immunogen
Recombinant Protein of Cystatin C of CST3
Modification
Unmodified/Total
Preparation and Storage
Store at -20 degree C/1 year

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human heart. 1. Antibody was diluted at 1:100(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Fallopian tube. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Cystatin C Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Brain Tissue using Cystatin C Mouse mAb diluted at 1:200.)

WB (Western Blot)

(Western Blot analysis of Cystatin C protein using antibody diluted at 1:1000)

WB (Western Blot) (Western Blot analysis of Cystatin C protein using antibody diluted at 1:1000)
Related Product Information for anti-CST3 antibody
The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes the most abundant extracellular inhibitor of cysteine proteases, which is found in high concentrations in biological fluids and is expressed in virtually all organs of the body. A mutation in this gene has been associate
Product Categories/Family for anti-CST3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
146
NCBI Official Full Name
Cystatin-C
NCBI Official Synonym Full Names
cystatin C
NCBI Official Symbol
CST3
NCBI Official Synonym Symbols
ARMD11
NCBI Protein Information
cystatin-C; cystatin 3; cystatin-3; gamma-trace; post-gamma-globulin; bA218C14.4 (cystatin C); neuroendocrine basic polypeptide
UniProt Protein Name
Cystatin-C
UniProt Gene Name
CST3
UniProt Entry Name
CYTC_HUMAN

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Product Notes

The CST3 cst3 (Catalog #AAA30828) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cystatin C Mouse Monoclonal Antibody (7F11) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cystatin C can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), ELISA (EIA). IF: 1:50-200 WB: 1:1000-2000 IHC: 1:100-200. Researchers should empirically determine the suitability of the CST3 cst3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cystatin C, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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