IHC (Immunohistochemistry)
(Immunohistochemical staining of Cytochrome P450 aldosterone synthase in low sodium rat renal tissue using AAA14663.)
anti-Mouse Cytochrome P450 Aldosterone Synthase Monoclonal Antibody | anti-CYP11B2 antibody
Cytochrome P450 Aldosterone Synthase (CYP11b2)
Gene Names
CYP11B2; CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo
Reactivity
Mouse
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Supernatant
Synonyms
Cytochrome P450 Aldosterone Synthase, Antibody; Cytochrome P450 Aldosterone Synthase (CYP11b2); Anti -Cytochrome P450 Aldosterone Synthase (CYP11b2); anti-CYP11B2 antibody
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2Q2321
Specificity
Recognizes rat cytochrome p450 aldosterone synthase. Species Crossreactivity : Mouse.
Purity/Purification
Supernatant
Form/Format
Supplied as a liquid, 1 mg/ml sodium azide, 50% glycerol.
Applicable Applications for anti-CYP11B2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Dilution: Western Blot: A band is observed at ~48kD in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable.
Immunohistochemistry: 1:50-1:200 (ABC, enzymatic detection). Works well with STF fixed tissues that are paraffin embedded. Also works with formalin fixed paraffin embedded tissues but at much lower working dilutions. Recommended counterstain is Gill's Hematoxylin.
Immunocytochemistry: 1:20-1:200 on acetone fixed cells.
Optimal dilutions to be determined by researcher.
Other applications not yet tested.
Immunohistochemistry: 1:50-1:200 (ABC, enzymatic detection). Works well with STF fixed tissues that are paraffin embedded. Also works with formalin fixed paraffin embedded tissues but at much lower working dilutions. Recommended counterstain is Gill's Hematoxylin.
Immunocytochemistry: 1:20-1:200 on acetone fixed cells.
Optimal dilutions to be determined by researcher.
Other applications not yet tested.
Immunogen
Rat Aldo-1-MAP aa175-192, KVRQNARGSLTMDVQQSL-MAP.
Recommended Positive Control
Adrenal zona glomerulosa of rats on a 7-14 day low sodium diet where the gene is overexpressed.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for up to 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IHC (Immunohistochemistry)
(Immunohistochemical staining of Cytochrome P450 aldosterone synthase in low sodium rat renal tissue using AAA14663.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of Cytochrome P450 aldosterone synthase in low sodium rat renal tissue using AAA14663.)
Related Product Information for anti-CYP11B2 antibody
Aldosterone synthase (or 18-hydroxylase) is a steroid hydroxylase cytochrome P450 oxidase enzyme involved in the generation of aldosterone. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity.
Product Categories/Family for anti-CYP11B2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
cytochrome P450 11B2, mitochondrial
NCBI Official Synonym Full Names
cytochrome P450, family 11, subfamily B, polypeptide 2
NCBI Official Symbol
CYP11B2
NCBI Official Synonym Symbols
CPN2; ALDOS; CYP11B; CYP11BL; CYPXIB2; P450C18; P-450C18; P450aldo
NCBI Protein Information
cytochrome P450 11B2, mitochondrial; cytochrome P-450C18; aldosterone synthase; cytochrome P-450Aldo; steroid 11-beta-monooxygenase; steroid 11-beta/18-hydroxylase; aldosterone-synthesizing enzyme; steroid 18-hydroxylase, aldosterone synthase, P450C18, P450aldo; mitochondrial cytochrome P450, family 11, subfamily B, polypeptide 2; cytochrome P450, subfamily XIB (steroid 11-beta-hydroxylase), polypeptide 2
UniProt Protein Name
Cytochrome P450 11B2, mitochondrial
UniProt Gene Name
CYP11B2
UniProt Synonym Gene Names
ALDOS
UniProt Entry Name
C11B2_HUMAN
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Product Notes
The CYP11B2 cyp11b2 (Catalog #AAA14663) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 Aldosterone Synthase (CYP11b2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 Aldosterone Synthase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Dilution: Western Blot: A band is observed at ~48kD in adrenal zona glomerulosa mitochondrial preparations. The enzyme is expressed best in adrenal zona glomerulosa tissues, expression in kidney is virtually undetectable. Immunohistochemistry: 1:50-1:200 (ABC, enzymatic detection). Works well with STF fixed tissues that are paraffin embedded. Also works with formalin fixed paraffin embedded tissues but at much lower working dilutions. Recommended counterstain is Gill's Hematoxylin. Immunocytochemistry: 1:20-1:200 on acetone fixed cells. Optimal dilutions to be determined by researcher. Other applications not yet tested. Researchers should empirically determine the suitability of the CYP11B2 cyp11b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 Aldosterone Synthase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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