Mouse E Coli K99 pili Monoclonal Antibody
E. coli K99 Pilus Monoclonal Antibody
Applications
ELISA
Purity
Protein G Purified
Synonyms
E Coli K99 pili, Antibody; E. coli K99 Pilus Monoclonal Antibody; Anti-E. coli K99 pili; E. coli K99 pili, Anti-E coli K99 Pilis Monoclonal Antibody; anti-E Coli K99 pili antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
K9912-444.5
Specificity
These antibodies recognize K99+ E. coli. They do not cross-react with K99-negative E. coli (B41), E. coli K88ac, E. coli 987P, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Enterobacter aerogenes, Citrobacter freundii, Klebsiella pneumoniae, or Strep. agalactiae.
Purity/Purification
Protein G Purified
Form/Format
Protein G-purified antibody in PBS, pH 7.4.
Concentration
1.1 mg/ml in PBS, pH7.4 (varies by lot)
Applicable Applications for anti-E Coli K99 pili antibody
These antibodies have been qualified for use in ELISA to detect K99+ E.coli.
Application Notes
Dilute in PBS or medium that is identical to that used in the assay system.
Appearance
Clear
Immunogen
Purified E.coli K99 (B44) pili
Preparation and Storage
These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Related Product Information for anti-E Coli K99 pili antibody
BACKGROUND: Bovine enteric colibacillosis is an economically important disease in neonatal calves caused by enterotoxigenic E. coli (ETEC) which adhere to intestinal cells using pili. Most ETEC isolated from cattle use K99 pili to bind to enterocytes of the small intestine. K99 pili are primarily composed of a repeating 18.2kDa major subunit which is responsible for binding to host glycoprotein receptor, N-glycolylneuraminic acid-GM3. A 7.1kb operon of eight genes on a plasmid encode the pili and other associated proteins responsible for regulation, transport, and assembly of K99 pili.
Product Categories/Family for anti-E Coli K99 pili antibody
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Product Notes
The E Coli K99 pili (Catalog #AAA11717) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's E Coli K99 pili can be used in a range of immunoassay formats including, but not limited to, These antibodies have been qualified for use in ELISA to detect K99+ E.coli. Dilute in PBS or medium that is identical to that used in the assay system. Researchers should empirically determine the suitability of the E Coli K99 pili for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E Coli K99 pili, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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