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Rat anti-Mouse EMR1 Monoclonal Antibody | anti-EMR1 antibody
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (MaxLight 750)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, immunoassay, Radioimmunoassay, Western Blot
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Synonyms
EMR1, Antibody; EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (MaxLight 750); EGF-like Module Receptor 1; F4/80 Antigen; anti-EMR1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9E38
Specificity
Recognizes mouse F4/80.
Purity/Purification
Purified by protein G affinity chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight750.
Applicable Applications for anti-EMR1 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Radioimmunoassay (RIA), Western Blot (WB)
Immunogen
Thioglycollate stimulated peritoneal macrophages from C57/BL mice.
Conjugate
MaxLight750
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN: ALEXA 405)
Application Data
(Staining of mouse J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN: RPE)
Application Data
(Staining of J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN:Low Endotoxin)
Application Data
(Staining of J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN:Biotin)
Application Data
(Frozen mouse spleen, using STAR72 as the secondary)
Application Data
(Staining of J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN:Biotin)
Product Categories/Family for anti-EMR1 antibody
References
1. Austyn, J.M. and Gordon, S. (1981) F4/80, a monoclonal antibody directed specifically against the mouse macrophage. Eur. J. Immunol. 11: 805-815, 2. Hume, D.A., Perry, V.H., and Gordon, S. (1984) The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia. The Anatomical Record 210: 503-512, 3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80. J. Exp. Med. 161: 475-489, 4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues. Curr. Top. Micro. Immunol. 181: 1-37, 5. Warschkau, H. and Kiderlen, A.F. (1999) A monoclonal antibody directed against the murine macrophage surface molecule F4/80 modulates natural immune response to Listeria monocytogenes. J. Immunol.163: 3409-3416, 6. Lin, H. H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance. J. Exp. Med. 201: 1615-1625, 7. Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor Blood. 105: 3737-3742, 8. F. X. Pizza et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice J. Physiol. 562:899-913, 9. Metwali, A. et al (2004) Cutting Edge: Hemokinin Has Substance P-Like Function and Expression in Inflammation The Journal of Immunology, 172: 6528-6532, 10. Kathryn J Moore et al (2009) Divergent Responses to LPS and Bacteria in CD14-Deficient Murine Macrophages. The Journal of Immunology, 165: 4272-4280
