Application Data
(Extracts from transduced 293fENL and HEK293 parental cells blotted with AAA11719)
Mouse ENL Monoclonal Antibody | anti-ENL antibody
Anti-ENL Monoclonal Antibody
Applications
Immunoblot, Western Blot
Purity
Purified by Protein G
Synonyms
ENL, Antibody; Anti-ENL Monoclonal Antibody; Anti-ENL; anti-ENL antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3.1.1
Specificity
This antibody recognizes human ENL.
Purity/Purification
Purified by Protein G
Form/Format
Clear Liquid ; PBS, pH 7.4
Concentration
0.65mg/mL (varies by lot)
Sequence Length
152
Applicable Applications for anti-ENL antibody
Immunoblot (IB)
Application Notes
Immunoblotting: Use at 1-5ug/ml. A band of ~62kDa is detected.
Dilute in PBS or medium that is identical to that used in the assay system.
Dilute in PBS or medium that is identical to that used in the assay system.
Immunogen
Maltose-binding fusion protein containing ENL amino acids 414-472 (Accession no. NP_005925).
Preparation and Storage
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Application Data
(Extracts from transduced 293fENL and HEK293 parental cells blotted with AAA11719)
Application Data
(Extracts from transduced 293fENL and HEK293 parental cells blotted with AAA11719)
Related Product Information for anti-ENL antibody
The AF4 and ENL (Eleven nineteen Leukemia) protein families are the most frequent MLL fusion partners accounting for two-thirds of MLL-associated leukemia incidence. The ENL family includes ENL and AF9 and has structural homology to the yeast Anc1 protein. ENL is a nuclear protein that is capable of activating transcription from synthetic reporter genes in both lymphoid and myeloid cells as well as in yeast. Deletion muta-genesis demonstrated that the minimal portion of ENL required for activation of transcription was localized to its C-terminal 90 amino acids. This region is highly conserved between ENL and AF9 and is retained in all Hrx-ENL and Hrx-AF9 fusion proteins. Thus, the leukemogenic contribution and transcriptional activation potential of ENL colocalize to its highly conserved carboxy terminus.
Product Categories/Family for anti-ENL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
ENL
UniProt Protein Name
ENL
UniProt Gene Name
ENL
UniProt Entry Name
A0A0S7M1F4_9TELE
Similar Products
Product Notes
The ENL enl (Catalog #AAA11719) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ENL can be used in a range of immunoassay formats including, but not limited to, Immunoblot (IB). Immunoblotting: Use at 1-5ug/ml. A band of ~62kDa is detected. Dilute in PBS or medium that is identical to that used in the assay system. Researchers should empirically determine the suitability of the ENL enl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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