Application Data
(Diffuse and strong estrogen receptor expression in mucinous breast carcinoma, stained with anti-ER monospecific antibody. Formalin fixed, paraffin embedded human tissue (4 um section) stained according to related datasheet.)
anti-Human ER (Estrogen receptor) Monoclonal Antibody | anti-ER antibody
Anti - ER (Estrogen receptor)
Gene Names
ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1
Reactivity
Human
Applications
Immunohistochemistry
Synonyms
ER (Estrogen receptor), Antibody; Anti - ER (Estrogen receptor); anti-ER antibody
Reactivity
Human
Clonality
Monoclonal
Clone Number
S21-V
Specificity
Human
Form/Format
20 mM Tris-HCI, pH 8.0
Sequence Length
595
Applicable Applications for anti-ER antibody
Immunohistochemistry-Paraffin (IHC-p)
Application Notes
IHC-P: Ready to use
IHC-P Protocol- INSTRUCTION MANUAL
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer*, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 min.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual.
13. Wash 3 x 5 minutes with buffer A.
14.Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0): Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage.
IHC-P Protocol- INSTRUCTION MANUAL
1. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each.
3. Rinse in distilled water, 2 x 5 minutes.
4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
5. Wash in distilled water, 2 x 5 minutes.
6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer*, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes.
7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes.
8. Rinse in distilled water, 2 x 5 minutes.
9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 min.
10. CONCENTRATED:
Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber.
READY TO USE (RTU):
Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber.
11. Wash 3 x 5 minutes with buffer A.
12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual.
13. Wash 3 x 5 minutes with buffer A.
14.Apply the chromogen (DAB), 1 - 3 minutes.
15. Wash in water, 2 x 5 minutes.
16. Stain in hematoxylin for 5 minutes.
17. Wash in distilled water, 3 x 2 minutes.
18. Mount the slide for observation.
* Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0): Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage.
Immunogen
Peptide derived from C-terminal sequence of human estrogen receptor alpha. Antibody recognizes the epitope between Gln580 - Thr594.
Stabilizer
20 mg/ml BSA
Cellular Localization
Nuclear membrane, cytoplasm
Positive Control
Human breast tumor tissue
Preservative
0.05% NaN3
Precautions
1. We strongly recommend to use Primary Antibody Diluent, eventually alternative diluent (containing protease free BSA at the concentrations > 1mg/ml) for dilution of concentrated antibodies.
2. Centrifuge the vial before use.
3. Intended for research use in laboratories.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
2. Centrifuge the vial before use.
3. Intended for research use in laboratories.
4. Do not use after expiration date stamped on vial label.
5. Avoid contamination of the reagent.
6. Any discrepancies in the recommended procedures stated in the working protocol may affect the final results.
7. The reagent contains sodium azide (NaN3) which is highly toxic in higher concentrations. The concentration in the reagent (0.05%) is not considered as hazardous.
8. Disposal of waste material must be conducted in accordance with local regulations.
9. Wear appropriate Personal Protective Equipment to avoid contact with eyes and skin.
Ventana Instruction Manual
Short Aapplication Protocol For Ventana Benchmark Slide Staining System:
Procedure: U ultraView DAB
1. Deparafinization
2. Heating (72 °C) at the medium temperatures. Deparafinization.
3. Cell conditioning
4. ULTRA conditioner #1
5. Heating glass (95 °C), incubation 8 min. (Cell conditioner #1; buffer CC1).
6. ULTRA CC1 solution application – 52 min.
7. Antibody incubation temperature
8. Heating glass (36 °C), incubation 4 min.
9. Titration
10. Hand apply – primary antibody 100 ?l. Incubation 36 min.
11. ultraWash
12. Nuclear stain
13. Hematoxylin II application – one drop (nuclear stain). Cover and incubate 12 min.
14. After nuclear stain
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min
Procedure: U ultraView DAB
1. Deparafinization
2. Heating (72 °C) at the medium temperatures. Deparafinization.
3. Cell conditioning
4. ULTRA conditioner #1
5. Heating glass (95 °C), incubation 8 min. (Cell conditioner #1; buffer CC1).
6. ULTRA CC1 solution application – 52 min.
7. Antibody incubation temperature
8. Heating glass (36 °C), incubation 4 min.
9. Titration
10. Hand apply – primary antibody 100 ?l. Incubation 36 min.
11. ultraWash
12. Nuclear stain
13. Hematoxylin II application – one drop (nuclear stain). Cover and incubate 12 min.
14. After nuclear stain
15. Bluing reagent application, one drop. After nuclear stain, cover and incubate 4 min
Leica Bond Max Protocol
Instruction Manual: Short Application Protocol For Leica Bond Max Slide Staining System
Protocol F:
1. Visualization system: BOND Refine DS9800
2. Epitope retrieval / heating time / temperature: ER2 / 20 min. / 100 °C
3.Incubation of primary antibody / temperature: 30 min. / 20 °
Protocol F:
1. Visualization system: BOND Refine DS9800
2. Epitope retrieval / heating time / temperature: ER2 / 20 min. / 100 °C
3.Incubation of primary antibody / temperature: 30 min. / 20 °
Preparation and Storage
Store at +4°C. Do not freeze.
Application Data
(Diffuse and strong estrogen receptor expression in mucinous breast carcinoma, stained with anti-ER monospecific antibody. Formalin fixed, paraffin embedded human tissue (4 um section) stained according to related datasheet.)
Application Data
(Diffuse and strong estrogen receptor expression in mucinous breast carcinoma, stained with anti-ER monospecific antibody. Formalin fixed, paraffin embedded human tissue (4 um section) stained according to related datasheet.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Estrogen receptor
NCBI Official Synonym Full Names
estrogen receptor 1
NCBI Official Symbol
ESR1
NCBI Official Synonym Symbols
ER; ESR; Era; ESRA; ESTRR; NR3A1
NCBI Protein Information
estrogen receptor; ER-alpha; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha delta 4 +49 isoform; nuclear receptor subfamily 3 group A member 1; estrogen receptor alpha 3*,4,5,6,7*/822 isoform; estrogen receptor alpha delta 4*,5,6,7*/654 isoform; estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform; estrogen receptor alpha delta 3*,4,5,6,7*/819-2 isoform; estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform
UniProt Protein Name
Estrogen receptor
UniProt Gene Name
ESR1
UniProt Synonym Gene Names
ESR; NR3A1; ER
UniProt Entry Name
ESR1_HUMAN
Similar Products
Product Notes
The ER esr1 (Catalog #AAA14931) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti - ER (Estrogen receptor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ER (Estrogen receptor) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-p). IHC-P: Ready to use IHC-P Protocol- INSTRUCTION MANUAL 1. Deparaffinize the section in 3 changes of xylene, 10 minutes each. 2. Wash the section in 96%, 80% and 70% ethyl alcohol for 10 minutes each. 3. Rinse in distilled water, 2 x 5 minutes. 4. Block the endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes. 5. Wash in distilled water, 2 x 5 minutes. 6. For antigen retrieval: Immerse the slide in Tris-EDTA buffer*, pH 9.0 and incubate at 95-97ºC in water bath for 25 minutes. 7. Remove the staining to room temperature and let the slide to cool (in Tris-EDTA buffer, pH 9.0) for 15 minutes. 8. Rinse in distilled water, 2 x 5 minutes. 9. Wash in PBS (phosphate buffer saline, pH 7.0-7.5) supplemented with 0.05% of Tween-20 (buffer A), 2 x 5 min. 10. CONCENTRATED: Incubate the section with primary antibody at the dilution 1:100 - 1:200 for 1 hour in the closed wet chamber. READY TO USE (RTU): Incubate the section with primary antibody (ready to use) for 1 hour in a closed wet chamber. 11. Wash 3 x 5 minutes with buffer A. 12. Apply the secondary antibody (the protocol depends on the supplier), and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). Micropolymer-HRP detection kit rabbit/mouse dual. 13. Wash 3 x 5 minutes with buffer A. 14.Apply the chromogen (DAB), 1 - 3 minutes. 15. Wash in water, 2 x 5 minutes. 16. Stain in hematoxylin for 5 minutes. 17. Wash in distilled water, 3 x 2 minutes. 18. Mount the slide for observation. * Tris-EDTA Buffer (10mM Tris Base, 1mM EDTA solution, 0.05% Tween-20, pH 9.0): Tris ----------- 1.21 g; EDTA ---------- 0.37 g; Distilled water ------------ 1000 ml Mix to dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1M HCl and then add 0.5 ml of Tween-20 and mix well. Adjust the final volume to 1 liter with distilled water. Store this solution at room temperature for 3 months or at +4ºC for longer storage. Researchers should empirically determine the suitability of the ER esr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ER (Estrogen receptor), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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