F4/80 recombinant antibody

Rabbit anti-F4/80 Recombinant Monoclonal Antibody [BLR209K]

Cat. Num. AAA23866

• Gene Names: Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Purified
• Synonyms: F4/80, Recombinant Antibody; Rabbit anti-F4/80 Recombinant Monoclonal Antibody [BLR209K]; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; TM7LN3; Ly71; Gpf480; F4/80; EMR1 hormone receptor; Emr1; EGF-TM7; EGF-like module receptor 1; DD7A5-7; cell surface glycoprotein F4/80; adhesion G protein-coupled receptor E1; EGF-like module-containing mucin-like hormone receptor-like 1; Adhesion G protein-coupled receptor E1; F4/80 recombinant antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Clone Number: BLR209K
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for F4/80 recombinant antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000 (3-8% Tris-acetate gel recommended); IP: 6 ul/mg lysate; IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Immunogen: Residues 28-225 (ECD)
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse F4/80 by western blot. Samples: Whole cell lysate (5 ug) from RAW 264.7, NIH 3T3, Ba/F3, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-F4/80 recombinant monoclonal antibody (AAA23866 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation)

(Detection of mouse F4/80 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from RAW 264.7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-F4/80 recombinant monoclonal antibody (AAA23866 lot 1) used for IP at 6 ul/mg lysate. F4/80 was also immunoprecipitated by a second antibody against a different epitope of F4/80 (BL-2189A-1B8). For blotting immunoprecipitated F4/80, AAA23866 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry)

(Detection of mouse F4/80 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-F4/80 recombinant monoclonal antibody (AAA23866 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of mouse F4/80 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-F4/80 recombinant monoclonal antibody (AAA23866 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry)

(Detection of mouse F4/80 by immunohistochemistry. Sample: FFPE section of mouse liver. Antibody: Rabbit anti-F4/80 recombinant monoclonal antibody (AAA23866 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of mouse F4/80 by immunocytochemistry. Sample: FFPE section of RAW 264.7 cells. Antibody: Rabbit anti-F4/80 recombinant monoclonal antibody (AAA23866 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry)

(Detection of mouse F4/80 by immunocytochemistry. Sample: FFPE section of J774A1 cells. Antibody: Rabbit anti-F4/80 recombinant monoclonal antibody (AAA23866 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

Related Product Information for F4/80 recombinant antibody

Adhesion G protein-coupled receptor E1 is also known as F4/80. It is an orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. In mouse, F4/80 is a well-characterized and extensively referenced macrophage marker. It is also known as Ly71 and EMR1, and is part of the EGF-TM7 family. It is a 160 kDa glycoprotein that shares 68% amino acid identity with human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1).

Product Categories/Family for F4/80 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 33859546
• NCBI GeneID: 13733
• NCBI Accession #: NP_034260.1
• NCBI GenBank Nucleotide #: NM_010130.4
• UniProt Accession #: Q61549
• Molecular Weight: 102,130 Da
• NCBI Official Full Name: EGF-like module-containing mucin-like hormone receptor-like 1
• NCBI Official Synonym Full Names: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• NCBI Official Symbol: Emr1
• NCBI Official Synonym Symbols: Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
• NCBI Protein Information: EGF-like module-containing mucin-like hormone receptor-like 1; EGF-like module receptor 1; EMR1 hormone receptor; cell surface glycoprotein F4/80; lymphocyte antigen 71
• UniProt Protein Name: EGF-like module-containing mucin-like hormone receptor-like 1
• UniProt Gene Name: Emr1
• UniProt Synonym Gene Names: Gpf480
• UniProt Entry Name: EMR1_MOUSE