Mouse anti-Human FOLR1 Monoclonal Antibody | anti-FOLR1 antibody

FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)

Cat. Num. AAA14691

• Gene Names: FOLR1; FBP; FOLR
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Immunocytochemistry, Western Blot
• Purity: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Synonyms: FOLR1, Antibody; FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18); Anti -FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18); anti-FOLR1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9H125
• Specificity: Recognizes human FOLR1. In Direct ELISA, shows no crossreactivity with recombinant human FOLR2, 3 or 4.
• Purity/Purification: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
• Form/Format: Supplied as lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
• Concentration: 0.5mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for anti-FOLR1 antibody: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.
• Application Notes: Recommended Dilution:; ELISA (Capture): 2-8ug/ml; Flow Cytometry: 0.25ug labels/10e6 cells. Tested in MCF-7 cells.; Western Blot: 2ug/ml detects human FOLR1 in human cortex tissue and HeLa cells under non-reducing conditions only.; Immunocytochemistry: 8-25ug/ml. Tested on immersion fixed MCF-7 cells.; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Recombinant protein corresponding to Arg25-Met233 of human FOLR1 expressed in CHO cells (P15328). Species Sequence Homology: mouse, rat; 83%
• Grade: Affinity Purified
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. . For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

FCM (Flow Cytometry)

(Flow Cytometry detection of FOLR1 in MCF-7 human cell line using AAA14691. MCF-7 human breast cancer cell line was stained with AAA14691 (filled histogram) or isotype control antibody (open histogram), followed byPhycoerythrin-conjugated Anti-MouseIgG Secondary Antibody.)

Application Data

(Detection of FOLR1 in MCF-7 human cell line. FOLR1 was detected in immersion fixed MCF-7 human breast cancer cell line using AAA14691 (10ug/ml) for 3 hours at RT. Cells were stained with the DayLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and)

WB (Western Blot)

(Western Blot detection of human FOLR1 using AAA14691. Lysates were prepared from Hela cells in non-reducing sample buffer, resolved by SDS-PAGE (30ug total protein/lane), and transferred to an Immobilon-P membrane. The blot was developed with AAA14691 (2ug/ml) and chemiluminescent detection substrate.)

Related Product Information for anti-FOLR1 antibody

FOLR1 is a 37-42kD protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine and histidine breakdown. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up- regulated on many carcinomas. It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk.

Product Categories/Family for anti-FOLR1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 9257213
• NCBI GeneID: 2348
• NCBI Accession #: NP_057941.1
• NCBI GenBank Nucleotide #: NM_016729.2
• UniProt Accession #: P15328; Q53EW2; Q6FGT8; Q6LC90; Q9UCT2
• NCBI Official Full Name: folate receptor alpha
• NCBI Official Synonym Full Names: folate receptor 1 (adult)
• NCBI Official Symbol: FOLR1
• NCBI Official Synonym Symbols: FBP; FOLR
• NCBI Protein Information: folate receptor alpha; FR-alpha; KB cells FBP; folate binding protein; folate receptor, adult; adult folate-binding protein; ovarian tumor-associated antigen MOv18
• UniProt Protein Name: Folate receptor alpha
• UniProt Gene Name: FOLR1
• UniProt Synonym Gene Names: FOLR; FR-alpha; FBP
• UniProt Entry Name: FOLR1_HUMAN