WB (Western Blot)
(Western Blot analysis for galactocerebroside in PC12 lysate. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with AAA14685 at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Galactocerebroside (~75kD).)
Mouse Galactocerebroside Monoclonal Antibody | anti-GALC antibody
Mouse anti-Bovine Galactocerebroside
Applications
ELISA, Immunohistochemistry, Immunocytochemistry
Purity
Purified by Protein A affinity chromatography.
Synonyms
Galactocerebroside, Antibody; Mouse anti-Bovine Galactocerebroside; Anti -Galactocerebroside; anti-GALC antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG3
Clone Number
2Q65
Specificity
Recognizes bovine Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Binds specifically with oligodendrocytes and Schwann cells. Crossreacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No crossreactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Species Crossreactivity: human, rat, rabbit and mouse.
Purity/Purification
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.09% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-GALC antibody
ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
ELISA: Used with purified galactocerebrosides
Immunohistochemistry: 0.5-10ug/ml on formalin-fixed frozen sections. Cannot be used on paraffin-embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry: 0.5-10ug/ml on 4% paraformaldehyde, acetic acid or ethanol-fixed cultured cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies -
Immunocytochemistry: IgG, F(ab')2 (FITC) Pab Gt x Mo, IgG F(ab')2 (HRP) Pab Gt x Mo
Western Blot: Please inquire.
Immunohistochemistry: 0.5-10ug/ml on formalin-fixed frozen sections. Cannot be used on paraffin-embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry: 0.5-10ug/ml on 4% paraformaldehyde, acetic acid or ethanol-fixed cultured cells.
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies -
Immunocytochemistry: IgG, F(ab')2 (FITC) Pab Gt x Mo, IgG F(ab')2 (HRP) Pab Gt x Mo
Western Blot: Please inquire.
Immunogen
Synaptic plasma membranes from bovine hippocampus (Accession# NM_000153.2).
Positive Control
Neonatal cortex
Grade
Affinity Purified
Preparation and Storage
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
WB (Western Blot)
(Western Blot analysis for galactocerebroside in PC12 lysate. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with AAA14685 at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Galactocerebroside (~75kD).)
WB (Western Blot)
(Western Blot analysis for galactocerebroside in PC12 lysate. PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with AAA14685 at 1:500 dilution. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein Galactocerebroside (~75kD).)
Related Product Information for anti-GALC antibody
Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.
Product Categories/Family for anti-GALC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
galactocerebrosidase isoform d
NCBI Official Synonym Full Names
galactosylceramidase
NCBI Official Symbol
GALC
NCBI Protein Information
galactocerebrosidase; GALCERase; galactosylceraminidase; galactocerebroside beta-galactosidase; galactosylceramide beta-galactosidase
UniProt Protein Name
Galactocerebrosidase
UniProt Gene Name
GALC
UniProt Synonym Gene Names
GALCERase
UniProt Entry Name
GALC_HUMAN
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Product Notes
The GALC galc (Catalog #AAA14685) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Galactocerebroside can be used in a range of immunoassay formats including, but not limited to, ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC). ELISA: Used with purified galactocerebrosides Immunohistochemistry: 0.5-10ug/ml on formalin-fixed frozen sections. Cannot be used on paraffin-embedded tissue sections since the antigen is denatured during embedding and paraffin removal. Immunocytochemistry: 0.5-10ug/ml on 4% paraformaldehyde, acetic acid or ethanol-fixed cultured cells. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies - Immunocytochemistry: IgG, F(ab')2 (FITC) Pab Gt x Mo, IgG F(ab')2 (HRP) Pab Gt x Mo Western Blot: Please inquire. Researchers should empirically determine the suitability of the GALC galc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galactocerebroside, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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