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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000)

GAPDH recombinant antibody

Recombinant GAPDH Monoclonal Antibody

Gene Names
Gapdh; Gapd
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Protein A/G Purification
Synonyms
GAPDH; Monoclonal Recombinant Antibody; Recombinant GAPDH Monoclonal Antibody; BARS-38; cb609; G3PDH; GAPD; KNC-NDS6; OCAS; GAPDH recombinant antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
20B8
Purity/Purification
Protein A/G Purification
Form/Format
TBS with 0.05% sodium azide, 0.05% protective protein and 40% glycerol, pH7.4
Concentration
1mg/mL (varies by lot)
Applicable Applications for GAPDH recombinant antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:20000-1:100000
IHC: 1:2000-1:4000
IF: 1:2000-1:4000
Immunogen
Recombinant protein within Mouse GAPDH
Preparation and Storage
Store at -20 degree C Valid for 12 months. Avoid freeze/thaw cycles. Protected from prolonged exposure to light.

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000)

IF (Immunofluorescence)

(Immunofluorescence analysis of U-2 OS cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Recombinant GAPDH Monoclonal Antibody at dilution of 1:1000)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded rat ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human colon cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon cancer using Recombinant GAPDH Monoclonal Antibody at dilution of 1:5000.)

WB (Western Blot)

(Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: C6 cell lysate, Lane 2: PC-12 cell lysate, Lane 3: Rat heart tissue lysate, Lane 4: Rat liver tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Rat brain tissue lysate, Lane 7:Rat stomach tissue lysate, Lane 8: Rat testis tissue lysate, Lane 9: Rat thymus tissue lysate, Lane 10: Rat skeletal muscle tissue lysate)

WB (Western Blot) (Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: C6 cell lysate, Lane 2: PC-12 cell lysate, Lane 3: Rat heart tissue lysate, Lane 4: Rat liver tissue lysate, Lane 5: Rat spleen tissue lysate, Lane 6: Rat brain tissue lysate, Lane 7:Rat stomach tissue lysate, Lane 8: Rat testis tissue lysate, Lane 9: Rat thymus tissue lysate, Lane 10: Rat skeletal muscle tissue lysate)

WB (Western Blot)

(Western blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. lane 1: mouse heart tissue lysate, lane 2: mouse liver tissue lysate, lane 3: mouse uterus tissue lysate, lane 4: mouse lung tissue lysate, lane 5: mouse kidney tissue lysate, lane 6: mouse brain tissue lysate, lane 7: mouse stomach tissue lysate)

WB (Western Blot) (Western blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. lane 1: mouse heart tissue lysate, lane 2: mouse liver tissue lysate, lane 3: mouse uterus tissue lysate, lane 4: mouse lung tissue lysate, lane 5: mouse kidney tissue lysate, lane 6: mouse brain tissue lysate, lane 7: mouse stomach tissue lysate)

WB (Western Blot)

(Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: A431 cell lysate,Lane 2: Caco-2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: K562 cell lysate, Lane 5: HeLa cell lysate, Lane 6: PC-3 cell lysate, Lane 7: Ramos cell lysate, Lane 8: TF-1 cell lysate, Lane 9: U20-S cell lysate, Lane 10: HL-60 cell lysate, Lane 11: HT-29 cell lysate, Lane 12: Raji cell lysate, Lane 13: HepG2 cell lysate)

WB (Western Blot) (Western Blot with Recombinant GAPDH Monoclonal Antibody at dilution of 1:160000. Lane 1: A431 cell lysate,Lane 2: Caco-2 cell lysate, Lane 3: Jurkat cell lysate, Lane 4: K562 cell lysate, Lane 5: HeLa cell lysate, Lane 6: PC-3 cell lysate, Lane 7: Ramos cell lysate, Lane 8: TF-1 cell lysate, Lane 9: U20-S cell lysate, Lane 10: HL-60 cell lysate, Lane 11: HT-29 cell lysate, Lane 12: Raji cell lysate, Lane 13: HepG2 cell lysate)
Related Product Information for GAPDH recombinant antibody
GAPDH is an enzyme of 36kDa that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. Because the GAPDH gene is often stably and constitutively expressed at high levels in most tissues and cells, it is commonly used as a loading control for western blot and as a control for qPCR.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,810 Da
NCBI Official Full Name
glyceraldehyde-3-phosphate dehydrogenase isoform 1
NCBI Official Synonym Full Names
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol
Gapdh
NCBI Official Synonym Symbols
Gapd
NCBI Protein Information
glyceraldehyde-3-phosphate dehydrogenase
UniProt Protein Name
Glyceraldehyde-3-phosphate dehydrogenase
UniProt Gene Name
Gapdh
UniProt Synonym Gene Names
Gapd; GAPDH
UniProt Entry Name
G3P_MOUSE

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Product Notes

The GAPDH gapdh (Catalog #AAA22336) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant GAPDH Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:20000-1:100000 IHC: 1:2000-1:4000 IF: 1:2000-1:4000. Researchers should empirically determine the suitability of the GAPDH gapdh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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