Mouse anti-Human GFAP Monoclonal Antibody | anti-GFAP antibody

GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody [Clone GFAP/6879]

Cat. Num. AAA23955

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Synonyms: GFAP, Antibody; GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody [Clone GFAP/6879]; GFAP (Astrocyte & Neural Stem Cell Marker); Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP); anti-GFAP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2c, kappa
• Clone Number: GFAP/6879
• Specificity: This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-GFAP antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Western Blot (WB): 2-4 ug/ml; Immunohistochemistry (IHC): 1-2ug/ml; IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95°C followed by cooling at RT for 20 minutes); ; Optimal dilution for a specific application should be determined.
• Immunogen: Recombinant fragment (around aa 150-250) of human GFAP protein (exact sequence is proprietary)
• Cellular Localization: Cytoplasm
• Positive Control: Human brain or astrocytoma.
• Research Areas: Cardiovascular, Endothelial Cell Marker, Neural Stem Cells, Neuroinflammation, Neuroscience, Signal Transduction
• Preparation and Storage: Antibody without azide: store at -20 to -80°C. Non-hazardous. No MSDS required.

IHC (Immunohistchemistry-Formalin)

(Formalin-fixed, paraffin-embedded human cerebellum stained with GFAPMouse Monoclonal Antibody (GFAP/6879). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.)

SDS-PAGE

(SDS-PAGE Analysis PurifiedGFAP Mouse Monoclonal Antibody (GFAP/6879). Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot)

(Western Blot Analysis of human brain tissue lysate using GFAP Mouse Monoclonal Antibody (GFAP/6879).)

Application Data

(Analysis of Protein Array containing more than 19,000 full-length human proteinsusing Monospecific Mouse Monoclonal Antibody to GFAP (GFAP/6879). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/6879))

IHC (Immunohistochemistry-Formalin)

(Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP Mouse Monoclonal Antibody (GFAP/6879).)

Related Product Information for anti-GFAP antibody

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

NCBI and Uniprot Product Information

• NCBI GI #: 196115290
• NCBI GeneID: 2670
• NCBI Accession #: NP_001124491.1
• NCBI GenBank Nucleotide #: NM_001131019.2
• UniProt Accession #: P14136; Q53H98; Q5D055; Q6ZQS3; Q7Z5J6; Q7Z5J7; Q96KS4; Q96P18; Q9UFD0; B2RD44; D3DX59
• Molecular Weight: ~50kDa
• NCBI Official Full Name: glial fibrillary acidic protein isoform 2
• NCBI Official Synonym Full Names: glial fibrillary acidic protein
• NCBI Official Symbol: GFAP
• NCBI Protein Information: glial fibrillary acidic protein
• UniProt Protein Name: Glial fibrillary acidic protein
• UniProt Gene Name: GFAP
• UniProt Synonym Gene Names: GFAP
• UniProt Entry Name: GFAP_HUMAN