SDS-PAGE
(SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2596).Confirmation of Integrity and Purity of Antibody)
Mouse GFAP Monoclonal Antibody | anti-GFAP antibody
GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
Synonyms
GFAP, Antibody; GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody; Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein; anti-GFAP antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
SPM507
Specificity
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Purity/Purification
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.
Form/Format
Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide.
Sequence Length
431
Applicable Applications for anti-GFAP antibody
Flow Cytometry (FC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Cellular Localization
Cytoplasmic
Immunogen
GFAP isolated from pig spinal cord
Positive Control
T98G cells (FC), Brain Iysate (WB),Brain or Astrocytoma (IHC)
Human Chromosome Location
17q21.31
Preparation and Storage
Antibody with azide , store at 2°C to 8°C
Non hazardous
No MSDS
Non hazardous
No MSDS
SDS-PAGE
(SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2596).Confirmation of Integrity and Purity of Antibody)
SDS-PAGE
(SDS-PAGE Analysis Purified EGFR Mouse Monoclonal Antibody (GFR/2596).Confirmation of Integrity and Purity of Antibody)
WB (Western Blot)
(Western Blot Analysis of Human Brain Tissue Lysate using GFAP Mab (SPM507).)
WB (Western Blot)
(Western Blot Analysis of Human Brain Tissue Lysate using GFAP Mab (SPM507).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (SPM507).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (SPM507).)
References
Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369. Debus E, et. al. 1983, Differentiation, 25(2):193-203
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~50kDa
NCBI Official Full Name
glial fibrillary acidic protein isoform 2
NCBI Official Synonym Full Names
glial fibrillary acidic protein
NCBI Official Symbol
GFAP
NCBI Official Synonym Symbols
ALXDRD
NCBI Protein Information
glial fibrillary acidic protein
UniProt Protein Name
Glial fibrillary acidic protein
UniProt Gene Name
GFAP
UniProt Synonym Gene Names
GFAP
UniProt Entry Name
GFAP_HUMAN
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Product Notes
The GFAP gfap (Catalog #AAA13808) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody reacts with Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's GFAP can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. Flow Cytometry (1-2ug/million cells) Immunofluorescence (1-2ug/ml) Western Blot (1-2ug/ml) Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the GFAP gfap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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