Mouse anti-Human, Rat GRP78 BiP Monoclonal Antibody | anti-HSPA5 antibody

GRP78 BiP (9C7) Mouse mAb

Cat. Num. AAA23990

• Gene Names: HSPA5; BIP; MIF2; GRP78
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: GRP78 BiP, Antibody; GRP78 BiP (9C7) Mouse mAb; HSPA5; GRP78;MIF2; BIP; Heat shock 70kDa protein 5; anti-HSPA5 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9C7-8D10-7A5
• Specificity: The GRP78/Bip mouse monoclonal antibody can recognizeendogenous GRP78/Bip proteins.
• Purity/Purification: Affinity purified
• Form/Format: PBS (pH7.4) containing with 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-HSPA5 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1;000-2;000; IHC: 1:100-200; (Optimal dilutions should be determined by the end user)
• Immunogen: Synthetic peptide conjugated to KLH.
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Cytoplasm, Endoplasmic reticulum
• Preparation and Storage: Store at 4 degree C short term. Store at -20 degree C long term. Avoid freeze/thaw cycle.

IP (Immunoprecipitation)

(Immunoprecipitation analysis of Hela cell lysates using Bip/GRP78 (9C7) Mouse mAb.)

ICC (Immunocytochemistry)

(Immunocytochemistry staining of HeLa cells using Bip/GRP78 (9C7) Mouse mAb(dilution 1:100).)

WB (Western Blot)

(Western blot detection of Bip/GRP78 in Jurkat,MCF7,Hela cell lysates using Bip/GRP78 (9C7) Mouse mAb(1:2000 diluted). Predicted band size:78KDa. Observed band size:78KDa.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human Breast Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human Colon Caricnoma using GRP78/BipMouse mAb diluted at 1:200.)

WB (Western Blot)

(Western blot analysis of 1)Hela, 2) Rat LiverTissue with GRP78/Bip Mouse mAb diluted at 1:2,000.)

Related Product Information for anti-HSPA5 antibody

Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

NCBI and Uniprot Product Information

• NCBI GI #: 16507237
• NCBI GeneID: 3309
• NCBI Accession #: NP_005338.1
• NCBI GenBank Nucleotide #: NM_005347.4
• UniProt Accession #: P11021; Q2EF78; Q9NPF1; Q9UK02; B0QZ61
• Molecular Weight: 72,333 Da
• NCBI Official Full Name: 78 kDa glucose-regulated protein
• NCBI Official Synonym Full Names: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)
• NCBI Official Symbol: HSPA5
• NCBI Official Synonym Symbols: BIP; MIF2; GRP78
• NCBI Protein Information: 78 kDa glucose-regulated protein; immunoglobulin heavy chain-binding protein; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78
• UniProt Protein Name: 78 kDa glucose-regulated protein
• UniProt Gene Name: HSPA5
• UniProt Synonym Gene Names: GRP78; GRP-78; BiP
• UniProt Entry Name: GRP78_HUMAN