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product-image-AAA21198_WB9.jpg WB (Western Blot) (Western Blot; Sample: Human Lung lysatePrimary Ab: 0.2ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

Mouse anti-Human, Rat Hedgehog Homolog, Sonic (SHH) Monoclonal Antibody | anti-SHH antibody

Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH)

Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Hedgehog Homolog, Sonic (SHH), Antibody; Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH); SHH/Hedgehog Homolog, Sonic; HHG1; HLP3; HPE3; SMMCI; SHHN; anti-SHH antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
D5
Specificity
The antibody is a mouse monoclonal antibody raised against SHH. It has been selected for its ability to recognize SHH in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-SHH antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.52ug/mL
IHC: 520ug/mL
ICC: 520ug/mL
Immunogen Type
Prokaryotic Recombinant proteins/RP
Sequence of Immunogen
Pro36~Ala193
Conjugation
Unconjugated
Preparation and Storage
Store at 2 degree C to 8 degree C for frequent use, 20 degree C for 24 months
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Western Blot; Sample: Human Lung lysatePrimary Ab: 0.2ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

product-image-AAA21198_WB9.jpg WB (Western Blot) (Western Blot; Sample: Human Lung lysatePrimary Ab: 0.2ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

WB (Western Blot)

(Western Blot; Sample: Rat Serum Primary Ab: 2ug/ml Mouse AntiHuman SHH Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

product-image-AAA21198_WB8.jpg WB (Western Blot) (Western Blot; Sample: Rat Serum Primary Ab: 2ug/ml Mouse AntiHuman SHH Antibody Second Ab: 0.2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

IHC (Immunohistochemistry)

(DAB staining on IHCP;Sample: Mouse Kidney Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

product-image-AAA21198_IHC7.jpg IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Mouse Kidney Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistchemistry)

(DAB staining on IHCP;Sample: Mouse Liver Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

product-image-AAA21198_IHC6.jpg IHC (Immunohistchemistry) (DAB staining on IHCP;Sample: Mouse Liver Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry)

(DAB staining on IHCP;Sample: Human Colon Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

product-image-AAA21198_IHC5.jpg IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Colon Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry)

(DAB staining on IHCP;Sample: Human Cerebrum Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

product-image-AAA21198_IHC4.jpg IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Cerebrum Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry)

(DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

product-image-AAA21198_IHC3.jpg IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Liver Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry)

(DAB staining on IHCP;Sample: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

product-image-AAA21198_IHC2.jpg IHC (Immunohistochemistry) (DAB staining on IHCP;Sample: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH AntibodySecond Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody(Catalog: SAA544Mu19))

IHC (Immunohistochemistry)

(DAB staining on IHCP; Sample: Human Cerebellum Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))

product-image-AAA21198_IHC.jpg IHC (Immunohistochemistry) (DAB staining on IHCP; Sample: Human Cerebellum Tissue; Primary Ab: 20ug/ml Mouse AntiHuman SHH Antibody Second Ab: 2ug/mL HRPLinked Caprine AntiMouse IgG Polyclonal Antibody (Catalog: SAA544Mu19))
Product Categories/Family for anti-SHH antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
462
NCBI Official Full Name
Sonic hedgehog protein
NCBI Official Synonym Full Names
sonic hedgehog
NCBI Official Symbol
SHH
NCBI Official Synonym Symbols
TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5
NCBI Protein Information
sonic hedgehog protein; sonic hedgehog homolog
UniProt Protein Name
Sonic hedgehog protein
UniProt Gene Name
SHH
UniProt Synonym Gene Names
SHH
UniProt Entry Name
SHH_HUMAN

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Product Notes

The SHH shh (Catalog #AAA21198) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Hedgehog Homolog, Sonic (SHH) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hedgehog Homolog, Sonic (SHH) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.52ug/mL IHC: 520ug/mL ICC: 520ug/mL. Researchers should empirically determine the suitability of the SHH shh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hedgehog Homolog, Sonic (SHH), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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