Mouse HER2 Monoclonal Antibody | anti-HER2 antibody

HER2 Monoclonal Antibody (11H9)

Cat. Num. AAA30802

• Gene Names: ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry
• Purity: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Synonyms: HER2, Antibody; HER2 Monoclonal Antibody (11H9); Receptor tyrosine-protein kinase erbB-2; ERBB2; HER2; MLN19; NEU; NGL; Metastatic lymph node gene 19 protein; MLN 19; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD340; anti-HER2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 11H9
• Specificity: The antibody detects endogenous ErbB-2/HER-2 proteins.
• Purity/Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
• Form/Format: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• Applicable Applications for anti-HER2 antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:2000-4000; IHC: 1:200; IF: 1:200
• Immunogen: Synthetic Peptide of HER2
• Modification: Unmodified/Total
• Preparation and Storage: Store at -20 degree C/1 year

IF (Immunofluorescence)

(Immunofluorescence analysis of Hela cell. 1.E2F-1 Polyclonal Antibody(red) was diluted at 1:200(4 degree overnight). HER2 Monoclonal Antibody(11H9)(green) was diluted at 1:200(4 degree overnight). 2. Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000(room temperature. 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000(room temperature. 50min).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.HER2 Monoclonal Antibody(11H9) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry)

(IHC staining of human breast cancer tissue. diluted at 1:200.)

Application Data

(Song. Xiaoping. et al. "A novel multi-target inhibitor harboring selectivity of inhibiting egfr T790M sparing wild-type EGFR." American journal of cancer research 7.9 (2017): 1884.)

WB (Western Blot)

(Western blot analysis of lysates from 1) Hela. 2) A431.3) MCF-7 cells. ?Green? primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. ?Red? Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot)

(Western blot analysis of lysates from 1) Hela. 2) A431.3) MCF-7 cells. (Green) primary antibody was diluted at 1:1000. 4 degree over night. secondary antibodywas diluted at 1:10000. 37 degree 1hour. (Red) Actin ? Polyclonal Antibody antibody was diluted at 1:5000 as loading control. 4 degree over night.secondary antibodywas diluted at 1:10000. 37 degree 1hour.)

WB (Western Blot)

(Western blot analysis of 1) Hela. 2) Mouse Brain. diluted at 1:4000.)

Related Product Information for anti-HER2 antibody

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding d

Product Categories/Family for anti-HER2 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 54792098
• NCBI GeneID: 2064
• NCBI Accession #: NP_001005862.1
• NCBI GenBank Nucleotide #: NM_001005862.1
• UniProt Accession #: P04626; Q14256; Q6LDV1; Q9UMK4; B2RZG3; B4DHN3
• Molecular Weight: 137,910 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2 isoform b
• NCBI Official Synonym Full Names: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• NCBI Official Symbol: ERBB2
• NCBI Official Synonym Symbols: NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived on
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• UniProt Gene Name: ERBB2
• UniProt Synonym Gene Names: HER2; MLN19; NEU; NGL; MLN 19
• UniProt Entry Name: ERBB2_HUMAN