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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit Histone H2B Monoclonal Antibody | anti-HIST1H2BK antibody
Histone H2B Antibody
Gene Names
HIST1H2BK; H2BK; H2B/S; H2BFT; H2BFAiii
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Histone H2B; Monoclonal Antibody; Histone H2B Antibody; H2B GL105 antibody; H2B histone family member O antibody; H2B histone family member S antibody; H2B.1 antibody; H2B.1 B antibody; H2B.b antibody; H2B.c antibody; H2B.d antibody; H2B.e antibody; H2B.f antibody; H2B.j antibody; H2B.q antibody; H2B/b antibody; H2B/c antibody; H2B/d antibody; H2B/e antibody; H2B/f antibody; H2B/j antibody; H2B/o antibody; H2B/q antibody; H2BFB antibody; H2BFC antibody; H2BFD antibody; H2BFE antibody; H2BFF antibody; H2BFJ antibody; H2BFO antibody; H2BFQ antibody; H2BFS antibody; HIRIP2 antibody; HIST1H2BB antibody; HIST1H2BD antibody; HIST1H2BH antibody; HIST1H2BL antibody; HIST1H2BM antibody; HIST1H2BN antibody; HIST2H2BE antibody; Histone H2B antibody; Histone H2B type 1 B antibody; Histone H2B type 1 D antibody; Histone H2B type 1 H antibody; Histone H2B type 1 L antibody; Histone H2B type 1 M antibody; Histone H2B type 1 N antibody; Histone H2B type 2 E antibody; histone protein antibody; anti-HIST1H2BK antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SD20-63
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HIST1H2BK antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Histone H2B on different lysates using anti-Histone H2B antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: PC12)
WB (Western Blot)
(Western blot analysis of Histone H2B on different lysates using anti-Histone H2B antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: PC12)
Related Product Information for anti-HIST1H2BK antibody
Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene, that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Product Categories/Family for anti-HIST1H2BK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,890 Da
NCBI Official Full Name
histone H2B type 1-K
NCBI Official Synonym Full Names
histone cluster 1, H2bk
NCBI Official Symbol
HIST1H2BK
NCBI Official Synonym Symbols
H2BK; H2B/S; H2BFT; H2BFAiii
NCBI Protein Information
histone H2B type 1-K
UniProt Protein Name
Histone H2B type 1-K
UniProt Gene Name
HIST1H2BK
UniProt Synonym Gene Names
H2BFT; HIRIP1; H2B K
UniProt Entry Name
H2B1K_HUMAN
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Product Notes
The HIST1H2BK hist1h2bk (Catalog #AAA30192) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H2B Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the HIST1H2BK hist1h2bk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H2B, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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