Mouse anti-Human HLA-A Monoclonal Antibody | anti-HLA-A antibody

HLA-A (MHC I) Mouse Monoclonal Antibody

Cat. Num. AAA13821

• Gene Names: HLA-B; AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HEL-S-83; HLA-DRB1; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602
• Reactivity: Human
• Applications: Flow Cytometry, Immunofluorescence
• Synonyms: HLA-A, Antibody; HLA-A (MHC I) Mouse Monoclonal Antibody; HLA-A; HLA class 1A; MHC class I HLA-A; anti-HLA-A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 108-2C5
• Specificity: HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of the alpha1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
• Sequence Length: 362

• Applicable Applications for anti-HLA-A antibody: Flow Cytometry (FC), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (1-2 ug/million cells); Immunofluorescence (1-2 ug/ml); Optimal dilution for a specific application should be determined
• Cellular Localization: Cell Surface
• Immunogen: Normal human peripheral blood lymphocytes
• Hu-Chromosome Location: 6q21.3
• Positive Control: Raji, THP-1 and Jurkat cells. Tonsil or lymph node.
• Preparation and Storage: Antibody with azide - store at 2°C to 8°C.; Non-hazardous.; No MSDS required.

FCM (Flow Cytometry)

(Flow Cytometric Analysis of Human Raji Cells. using HLA-Mouse Monoclonal Antibody followed by Goat anti-mouse IgG (Blue); Isotype control (Red).)

Application Data

(SDS-PAGE AnalysisPurified HLA-A Mouse Monoclonal Antibody (108-2C5))

References

Domenech N et al. Hum Immunol 1991, 30(2):140-6 Lozano F et al. Immunogenetics 1989, 30::50-3 Lozano F et al. Tissue Antigens 1990, 35:193-5 Young NT et al. Hum Immunol 1997, 52(1):1-11 Krensky AM et al Transplant Proc 1996, 28(6):3026-8 Hansen JA et al Hematol Oncol Clin North Am 1990, 4(3):507-515

NCBI and Uniprot Product Information

• NCBI GI #: 17986001
• NCBI GeneID: 3106
• NCBI Accession #: NP_005505.2
• NCBI GenBank Nucleotide #: NM_005514.7
• UniProt Accession #: P01889; Q29638; Q29681; Q29854; Q29861; Q31613; Q5SRJ2; Q9GIX1; Q9TP95; P18463; P10319; P18464; P18465; P30460; P30461; P30462; P30466; P30685; P30475
• Molecular Weight: ~41kDa
• NCBI Official Full Name: major histocompatibility complex, class I, B
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, B
• NCBI Official Symbol: HLA-B
• NCBI Official Synonym Symbols: AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HEL-S-83; HLA-DRB1; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602
• NCBI Protein Information: major histocompatibility complex, class I, B
• UniProt Protein Name: HLA class I histocompatibility antigen, B-7 alpha chain
• UniProt Gene Name: HLA-B
• UniProt Synonym Gene Names: HLAB
• UniProt Entry Name: 1B07_HUMAN