WB (Western Blot)
(HeLa cells were subjected to SDS PAGE followed by western blot with AAA27489 (HLA class I (HLA-A) Antibody) at dilution of 1:4000)
Mouse anti-Human HLA class I (HLA-A) Monoclonal Antibody | anti-HLA-A antibody
HLA class I (HLA-A) Mouse Monoclonal
Gene Names
HLA-A; HLAA; Aw-33; Aw-74; HLA-A11; HLA-A33; HLA-DQB1; HLA-DRB1
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Immunohistochemistry
Purity
>95% as determined by SDS-PAGEProtein A+G purification
Synonyms
HLA class I (HLA-A), Antibody; HLA class I (HLA-A) Mouse Monoclonal; HLA A, HLA class I, HLA class I (HLA-A), HLA class I ABC, HLA-A, MHC class I antigen A*3; anti-HLA-A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
0D3
Purity/Purification
>95% as determined by SDS-PAGE
Protein A+G purification
Protein A+G purification
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Sequence Length
365
Applicable Applications for anti-HLA-A antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:8000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Major histocompatibility complex, class I, A
Preparation and Storage
Store at -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
WB (Western Blot)
(HeLa cells were subjected to SDS PAGE followed by western blot with AAA27489 (HLA class I (HLA-A) Antibody) at dilution of 1:4000)
WB (Western Blot)
(HeLa cells were subjected to SDS PAGE followed by western blot with AAA27489 (HLA class I (HLA-A) Antibody) at dilution of 1:4000)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using AAA27489 (HLA class I (HLA-A) Antibody) at dilution of 1:200)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using AAA27489 (HLA class I (HLA-A) Antibody) at dilution of 1:200)
Related Product Information for anti-HLA-A antibody
Background: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44 kDa
NCBI Official Full Name
HLA class I histocompatibility antigen, A-1 alpha chain
NCBI Official Synonym Full Names
major histocompatibility complex, class I, A
NCBI Official Symbol
HLA-A
NCBI Official Synonym Symbols
HLAA; Aw-33; Aw-74; HLA-A11; HLA-A33; HLA-DQB1; HLA-DRB1
NCBI Protein Information
HLA class I histocompatibility antigen, A-1 alpha chain
UniProt Protein Name
HLA class I histocompatibility antigen, A-2 alpha chain
UniProt Gene Name
HLA-A
UniProt Synonym Gene Names
HLAA
UniProt Entry Name
1A02_HUMAN
Similar Products
Product Notes
The HLA-A hla-a (Catalog #AAA27489) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HLA class I (HLA-A) Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA class I (HLA-A) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunohistochemistry (IHC). WB: 1:1000-1:8000IHC: 1:20-1:200. Researchers should empirically determine the suitability of the HLA-A hla-a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA class I (HLA-A), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.