IHC (Immunohistochemistry)
(Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100)for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine (DAB))
Mouse anti-Human, Hsp90 Monoclonal Antibody | anti-Hsp90 antibody
Hsp90 antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human,
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
By protein-G affinity chromatography
Synonyms
Hsp90, Antibody; Hsp90 antibody; Heat shock protein HSP 90-alpha; HSP 86; Renal carcinoma antigen NY-REN-38; HSPC1; HSPCA; Heat shock protein 90-alpha Renal carcinoma antigen NY REN 38; D6S182; FLJ26984; FLJ31884; Heat shock 90kDa protein 1 alpha; heat shock protein 90kDa alpha (cytosolic); class A member 2; HSP84; HSP86; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90ALPHA; HSP90N; HSPCAL3; HSPCB; HSPN; LAP2; Lipopolysaccharide associated protein2; LPS associated protein 2; NY REN 38 antigen; hsp90; anti-Hsp90 antibody
Host
Mouse
Reactivity
Human,
Clonality
Monoclonal
Isotype
Mouse IgG2bheavy chain and k light
chain
Clone Number
4F10
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
732
Applicable Applications for anti-Hsp90 antibody
ELISA (EIA), WB (Cell lysate), Immunohistochemistry (IHC)
Application Notes
The antibody has been tested by ELISA, Western Blot, and Immunohistochemistry analysis to assure specifity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended diltuion range for Western Blot is 1:500~ 1:200 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western Blot is 1:1000 and Immunohistochemistry is 1:100.
Antigen Species
Human
Immunogen
Recombinant human Hsp90 (1-732aa) purified from E. coli
Clone Note
Anti-human Hsp90 mAb, clone 4F10, is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Hsp90 protein.
Preparation and Storage
Can be stored at 4°C for short term ( 1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C . Avoid repeated freezing and thawing cycles.
IHC (Immunohistochemistry)
(Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100)for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine (DAB))
IHC (Immunohistochemistry)
(Paraffin embedded sections of human urothelium were incubated with anti-human Hsp90 (1:100)for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detectedusing Diaminobenzidine (DAB))
FCM (Flow Cytometry)
(Flow cytometry analysis of HSP90in HepG2cell line,staining at 2-5ug for 1x106cellsredline). The secondary antibody used goat anti-mouse IgGAlexa fluor 488 conjugate. Isotype control antibody was mouse IgG(black line).)
FCM (Flow Cytometry)
(Flow cytometry analysis of HSP90in HepG2cell line,staining at 2-5ug for 1x106cellsredline). The secondary antibody used goat anti-mouse IgGAlexa fluor 488 conjugate. Isotype control antibody was mouse IgG(black line).)
WB (Western Blot)
(The Recombinant Human HSP90 (50ng) and Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection systemLane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate)
WB (Western Blot)
(The Recombinant Human HSP90 (50ng) and Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection systemLane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate)
Related Product Information for anti-Hsp90 antibody
Hsp90 is a human heat shock protein. In response to adverse change in their environment, cell from all organisms increase the expression of a class of proteins referred to as heat shock or stress protein. The Hsp90, a highly conserved stress-induced protein, is abundantly expressed in most tissues under non-stress conditions and is required for eukaryotic cell viability. Hsp90 is primarily a cytoplasmic protein and its function remains unknown. It exists in a dimeric form and has been observed to bind to several other cellular proteins such as retro-virus kinases, steroid receptor, heme-regulated protein kinase, actin and tubulin.
Product Categories/Family for anti-Hsp90 antibody
References
B-T, et al., (1984) Molecular & Cellular Biol 4: 2802-2810; Pritchard, K. A, et al., (2001) J Biol Chem 276:17621-17624; Miyamoto, A, et al., (2002) Nature 416:865-869
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
heat shock protein HSP 90-alpha isoform 2
NCBI Official Synonym Full Names
heat shock protein 90 alpha family class A member 1
NCBI Official Symbol
HSP90AA1
NCBI Official Synonym Symbols
EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
NCBI Protein Information
heat shock protein HSP 90-alpha
UniProt Protein Name
Heat shock protein HSP 90-alpha
UniProt Gene Name
HSP90AA1
UniProt Synonym Gene Names
HSP90A; HSPC1; HSPCA; HSP 86; HSP86; LAP-2; LPS-associated protein 2
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Product Notes
The Hsp90 hsp90aa1 (Catalog #AAA11726) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hsp90 antibody reacts with Human, and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp90 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Cell lysate), Immunohistochemistry (IHC). The antibody has been tested by ELISA, Western Blot, and Immunohistochemistry analysis to assure specifity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended diltuion range for Western Blot is 1:500~ 1:200 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western Blot is 1:1000 and Immunohistochemistry is 1:100. Researchers should empirically determine the suitability of the Hsp90 hsp90aa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp90, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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