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FCM (Flow Cytometry)
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Rabbit Huntingtin Monoclonal Antibody | anti-HTT antibody

Huntingtin Antibody

Gene Names
HTT; HD; IT15
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry
Purity
ProA affinity purified
Synonyms
Huntingtin, Antibody; Huntingtin Antibody; AI256365 antibody; C430023I11Rik antibody; HD antibody; HD protein antibody; HD_HUMAN antibody; HDH antibody; HTT antibody; Huntingtin antibody; HUNTINGTON CHOREA antibody; Huntington disease protein antibody; Huntington's disease protein homolog antibody; IT 15 antibody; IT15 antibody; OTTMUSP00000026909 antibody; ZHD antibody; anti-HTT antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB89-34
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-HTT antibody
Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)
Application Notes
WB: 1:500
IHC: 1:50-1:200
ICC: 1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of Huntingtin on A431 cell using anti-Huntingtin antibody at 1/500 dilution.)

Related Product Information for anti-HTT antibody
The huntingtin gene, also called the HTT or HD (Huntington disease) gene, is the IT15 ("interesting transcript 15") gene, which codes for a protein called the huntingtin protein. It is variable in its structure, as the many polymorphisms of the gene can lead to variable numbers of glutamine residues present in the protein. The mass of huntingtin protein is dependent largely on the number of glutamine residues it has, the predicted mass is around 350 kDa. Normal huntingtin is generally accepted to be 3144 amino acids in size. The exact function of this protein is not known, but it plays an important role in nerve cells. Within cells, huntingtin may be involved in signaling, transporting materials, binding proteins and other structures, and protecting against programmed cell death (apoptosis). The huntingtin protein is required for normal development before birth. It is expressed in many tissues in the body, with the highest levels of expression seen in the brain.
Product Categories/Family for anti-HTT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
347,603 Da
NCBI Official Full Name
huntingtin
NCBI Official Synonym Full Names
huntingtin
NCBI Official Symbol
HTT
NCBI Official Synonym Symbols
HD; IT15
NCBI Protein Information
huntingtin; huntington disease protein
UniProt Protein Name
Huntingtin
UniProt Gene Name
HTT
UniProt Synonym Gene Names
HD; IT15; HD protein
UniProt Entry Name
HD_HUMAN

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Product Notes

The HTT htt (Catalog #AAA30460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Huntingtin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Huntingtin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC). WB: 1:500 IHC: 1:50-1:200 ICC: 1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the HTT htt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Huntingtin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.