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IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Lung Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
Mouse Interleukin 6 Monoclonal Antibody | anti-IL6 antibody
Monoclonal Antibody to Interleukin 6 (IL6)
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Rattus norvegicus (Rat)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
Interleukin 6; Monoclonal Antibody; Monoclonal Antibody to Interleukin 6 (IL6); anti-IL6 antibody
Host
Mouse
Reactivity
Rattus norvegicus (Rat)
Clonality
Monoclonal
Isotype
IgG2a Kappa
Clone Number
C4
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-IL6 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.01-5ug/mL
Immunohistochemistry: 5-50ug/mL
Immunocytochemistry: 5-50ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-50ug/mL
Immunocytochemistry: 5-50ug/mL
Optimal working dilutions must be determined by end user.
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant IL6 (Thr21~Thr211) expressed in E.coli .
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Lung Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Lung Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Rat Pancreas Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistchemistry)
(DAB staining on IHC-P;Samples: Rat Pancreas Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Small Intestine Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Small Intestine Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Stomach Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Stomach Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Liver Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Liver Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Spleen Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
IHC (Immunohistochemistry)
(DAB staining on IHC-P;Samples: Rat Spleen Tissue;Primary Ab: 40ug/ml Mouse Anti-Rat IL6 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog:))
WB (Western Blot)
(Western Blot;Sample: Recombinant IL6, Rat.)
WB (Western Blot)
(Western Blot;Sample: Recombinant IL6, Rat.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
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Product Notes
The IL6 il6 (Catalog #AAA20825) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Interleukin 6 (IL6) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.01-5ug/mL Immunohistochemistry: 5-50ug/mL Immunocytochemistry: 5-50ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IL6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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