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FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit KDEL Monoclonal Antibody | anti-KDEL antibody
KDEL Antibody
Gene Names
KDELR1; ERD2; HDEL; PM23; ERD2.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
KDEL; Monoclonal Antibody; KDEL Antibody; ER lumen protein retaining receptor 1 antibody; ERD2.1 antibody; ERD21_HUMAN antibody; KDEL endoplasmic reticulum protein retention receptor 1 antibody; KDEL receptor 1 antibody; Kdelr1 antibody; Putative MAPK-activating protein PM23 antibody; anti-KDEL antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JB42-04
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-KDEL antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:100-1:400
ICC: 1:400-1:800
FC/FACS: 1:50-1:100
IHC: 1:100-1:400
ICC: 1:400-1:800
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with KDEL antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining KDEL in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining KDEL in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining KDEL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining KDEL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining KDEL in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-KDEL antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1, 000 dilution. Positive control�� Lane 1: Rat testis tissue Lane 2: Human placenta tissue Lane 3: Mouse testis tissue Lane 4: 293)
WB (Western Blot)
(Western blot analysis of KDEL on different lysates using anti-KDEL antibody at 1/1, 000 dilution. Positive control�� Lane 1: Rat testis tissue Lane 2: Human placenta tissue Lane 3: Mouse testis tissue Lane 4: 293)
Related Product Information for anti-KDEL antibody
Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrograde vesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus. When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor. Subsequently, this interaction allows the KDEL receptor to recruit a GTPase-activating protein (GAP) from the cytosol to membranes, which inactivates ARF1.
Product Categories/Family for anti-KDEL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
24,542 Da
NCBI Official Full Name
KDEL receptor
NCBI Official Synonym Full Names
KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
NCBI Official Symbol
KDELR1
NCBI Official Synonym Symbols
ERD2; HDEL; PM23; ERD2.1
NCBI Protein Information
ER lumen protein retaining receptor 1; KDEL receptor 1; putative MAPK-activating protein PM23; KDEL endoplasmic reticulum protein retention receptor 1
UniProt Protein Name
ER lumen protein retaining receptor 1
UniProt Gene Name
KDELR1
UniProt Synonym Gene Names
ERD2.1
UniProt Entry Name
ERD21_HUMAN
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Product Notes
The KDEL kdelr1 (Catalog #AAA30471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KDEL Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KDEL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:100-1:400 ICC: 1:400-1:800 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the KDEL kdelr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KDEL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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