<
IHC (Immunohistochemistry)
(LAG3 Antibody-Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
Mouse anti-Human LAG3 Monoclonal Antibody | anti-LAG3 antibody
LAG3 Mouse anti-Human Monoclonal (9F9) Antibody
Gene Names
LAG3; CD223
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Protein A purified
Synonyms
LAG3; Monoclonal Antibody; LAG3 Mouse anti-Human Monoclonal (9F9) Antibody; PathPlus LAG3 Antibody (clone 9F9); CD223 antigen; LAG-3; Protein FDC; CD223; FDC; Lymphocyte-activation gene 3; anti-LAG3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
9F9
Purity/Purification
Protein A purified
Form/Format
PBS, 0.02% Sodium Azide, 50% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LAG3 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P)
Target
Human LAG3
Immunogen
The extracellular domain of human LAG-3
Conjugation
Unconjugated
Preparation and Storage
Store at 4 degree C for 3 months and -20 degree C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
IHC (Immunohistochemistry)
(LAG3 Antibody-Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
IHC (Immunohistochemistry)
(LAG3 Antibody-Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
IHC (Immunohistchemistry)
(LAG3 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(LAG3 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(LAG3 Antibody-Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(LAG3 Antibody-Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(LAG3 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(LAG3 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))
ICC (Immunocytochemistry)
(LAG3 Antibody-Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 ug/ml. Red: LAG3 Antibody [9F9] Blue: DAPI staining)
ICC (Immunocytochemistry)
(LAG3 Antibody-Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 ug/ml. Red: LAG3 Antibody [9F9] Blue: DAPI staining)
ICC (Immunocytochemistry)
(LAG3 Antibody-Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 ug/ml. Green: LAG3 Antibody [9F9] Blue: DAPI staining)
ICC (Immunocytochemistry)
(LAG3 Antibody-Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 ug/ml. Green: LAG3 Antibody [9F9] Blue: DAPI staining)
FCM (Flow Cytometry)
(LAG3 Antibody-Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.)
FCM (Flow Cytometry)
(LAG3 Antibody-Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.)
Related Product Information for anti-LAG3 antibody
LAG3 antibody is an unconjugated mouse monoclonal antibody to human LAG3. Validated for ELISA, Flow, ICC, IF and IHC.
Lymphocyte activation gene-3 (LAG3, CD223) is membranous co-inhibitory cell surface receptor expressed on activated CD4 and CD8 T-cells that controls immune responses and acts as an immune checkpoint receptor. It is expressed on activated T-cells, natural killer cells, and plasmacytoid dendritic calls. LAG3 negatively regulates cell proliferation, activation, cytokine production and Treg cell mediated control of homeostasis of T cells. Lag-3 is expressed on exhausted effector tumor infiltrating lymphocytes and blockade has been demonstrated to improve anti-tumor CD8 T cell responses. It is the target of drug development to treat cancer, chronic viral infection and autoimmune disorders.
Lymphocyte activation gene-3 (LAG3, CD223) is membranous co-inhibitory cell surface receptor expressed on activated CD4 and CD8 T-cells that controls immune responses and acts as an immune checkpoint receptor. It is expressed on activated T-cells, natural killer cells, and plasmacytoid dendritic calls. LAG3 negatively regulates cell proliferation, activation, cytokine production and Treg cell mediated control of homeostasis of T cells. Lag-3 is expressed on exhausted effector tumor infiltrating lymphocytes and blockade has been demonstrated to improve anti-tumor CD8 T cell responses. It is the target of drug development to treat cancer, chronic viral infection and autoimmune disorders.
References
Immunity 2016, 44:989
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
525
NCBI Official Full Name
Lymphocyte activation gene 3 protein
NCBI Official Synonym Full Names
lymphocyte-activation gene 3
NCBI Official Symbol
LAG3
NCBI Official Synonym Symbols
CD223
NCBI Protein Information
lymphocyte activation gene 3 protein
UniProt Protein Name
Lymphocyte activation gene 3 protein
UniProt Gene Name
LAG3
UniProt Synonym Gene Names
FDC; LAG-3
UniProt Entry Name
LAG3_HUMAN
Similar Products
Product Notes
The LAG3 lag3 (Catalog #AAA21352) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The LAG3 Mouse anti-Human Monoclonal (9F9) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LAG3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P). Researchers should empirically determine the suitability of the LAG3 lag3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LAG3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
