Rabbit anti-Human, Mouse Lipocalin-2/NGAL Monoclonal Antibody | anti-LCN2 antibody

Lipocalin-2/NGAL Rabbit PolymAb

Cat. Num. AAA28588

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity purification
• Synonyms: Lipocalin-2/NGAL, Antibody; Lipocalin-2/NGAL Rabbit PolymAb; p25; 24p3; MSFI; NGAL; Lipocalin-2/NGAL; AW212229; NRL; Sip24; anti-LCN2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.

• Applicable Applications for anti-LCN2 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; IHC-P: 1:500-1:2000; IF/ICC: 1:200-1:400; ELISA: Recommended starting concentration is 1ug/mL.; Please optimize the concentration based on your specific assay requirements.
• Immunogen: Recombinant protein of human/mouse Lipocalin-2/NGAL.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

ICC (Immunocytochemistry)

(Confocal imaging of paraffin-embedded Human spleen tissue using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry)

(Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot)

(Western blot analysis of lysates from A-431 cells using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

WB (Western Blot)

(Western blot analysis of various lysates using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588)at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): Mouse brainExposure time: 10s.)

WB (Western Blot)

(Western blot analysis of various lysates using Lipocalin-2/NGAL Rabbit PolymAb® (AAA28588)at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): 293TExposure time: 10s.)

Related Product Information for anti-LCN2 antibody

This gene encodes a protein that belongs to the lipocalin family. Members of this family transport small hydrophobic molecules such as lipids, steroid hormones and retinoids. The protein encoded by this gene is a neutrophil gelatinase-associated lipocalin and plays a role in innate immunity by limiting bacterial growth as a result of sequestering iron-containing siderophores. The presence of this protein in blood and urine is an early biomarker of acute kidney injury. This protein is thought to be be involved in multiple cellular processes, including maintenance of skin homeostasis, and suppression of invasiveness and metastasis. Mice lacking this gene are more susceptible to bacterial infection than wild type mice.

Product Categories/Family for anti-LCN2 antibody

Cancer; Tumor biomarkers; Signal Transduction; Endocrine Metabolism; Lipid Metabolism; Immunology Inflammation; Cardiovascular; Lipids; Fatty Acids; Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 49457137
• NCBI GeneID: 3934/16819
• UniProt Accession #: P80188; P11672
• Molecular Weight: Calculated MW: 23kDa; Observed MW: 23kDa
• UniProt Protein Name: Neutrophil gelatinase-associated lipocalin
• UniProt Gene Name: LCN2
• UniProt Synonym Gene Names: HNL; NGAL; NGAL
• UniProt Entry Name: NGAL_HUMAN