Application Data
(Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase:HRP)
Mouse LIPOPROTEIN LIPASE Monoclonal Antibody | anti-LPL antibody
MOUSE ANTI LIPOPROTEIN LIPASE:Biotin
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
LIPOPROTEIN LIPASE, Antibody; MOUSE ANTI LIPOPROTEIN LIPASE:Biotin; anti-LPL antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5D2
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Biotin
Purified IgG conjugated to Biotin - liquid
Buffer Solution: Phosphate buffered saline
Purified IgG conjugated to Biotin - liquid
Buffer Solution: Phosphate buffered saline
Concentration
IgG concentration 0.1mg/ml (varies by lot)
Sequence Length
478
Applicable Applications for anti-LPL antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Western Blot: This item detects a band of approximately 53kDa using partially purified LPL from postheparin plasma.
ELISA: can be used in an indirect ELISA
ELISA: can be used in an indirect ELISA
Perservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Immunogen
Purified bovine milk lipoprotein lipase
RRID
AB_10889937
Target Species
Bovine
Species Cross-Reactivity
Reacts with: Rat, Human, Chicken, Guinea Pig
Does not react with: Mouse N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators.
Does not react with: Mouse N.B. Antibody reactivity and working conditions may vary between species. Cross reactivity is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators.
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt.
When thawed, aliquot the sample as needed.
Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
When thawed, aliquot the sample as needed.
Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Data
(Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase:HRP)
Application Data
(Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase:HRP)
Application Data
(Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Application Data
(Bovine lipoprotein lipase detected with Mouse anti lipoprotein lipase followed by Rabbit F(ab')2 anti Mouse IgG:HRP)
Related Product Information for anti-LPL antibody
Mouse anti Lipoprotein Lipase antibody, clone 5D2 specifically recognizes an epitope within amino acids 380-410 of lipoprotein lipase (LPL), a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues. A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. Mouse anti Lipoprotein Lipase antibody, clone 5D2 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions. Mouse anti Lipoprotein Lipase antibody, clone 5D2 has been shown to inhibit the activity of human LPL (Chang et al. 1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
lipoprotein lipase
NCBI Official Synonym Full Names
lipoprotein lipase<
NCBI Official Symbol
LPL
NCBI Protein Information
lipoprotein lipase
UniProt Protein Name
Lipoprotein lipase
UniProt Gene Name
LPL
UniProt Synonym Gene Names
LPL
UniProt Entry Name
LIPL_BOVIN
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Product Notes
The LPL lpl (Catalog #AAA12249) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's LIPOPROTEIN LIPASE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Western Blot: This item detects a band of approximately 53kDa using partially purified LPL from postheparin plasma. ELISA: can be used in an indirect ELISA. Researchers should empirically determine the suitability of the LPL lpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIPOPROTEIN LIPASE, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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