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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit LOX Monoclonal Antibody | anti-LOX antibody
LOX Antibody
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
LOX; Monoclonal Antibody; LOX Antibody; lox antibody; LYOX antibody; LYOX_HUMAN antibody; Lysyl oxidase antibody; MGC105112 antibody; Protein lysine 6 oxidase antibody; Protein-lysine 6-oxidase antibody; anti-LOX antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Monoclonal
Clone Number
JU30-23
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-LOX antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
IHC: 1:50-1:100
ICC: 1:100-1:500
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with LOX antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining LOX in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining LOX in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining LOX in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining LOX in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining LOX in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining LOX in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-LOX antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-LOX antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LOX antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LOX antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of LOX on Zebrafish tissue lysates using anti-LOX antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of LOX on Zebrafish tissue lysates using anti-LOX antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Mouse brain Lane 3: Human fetal brain)
WB (Western Blot)
(Western blot analysis of LOX on different lysates using anti-LOX antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Mouse brain Lane 3: Human fetal brain)
Related Product Information for anti-LOX antibody
The lysyl oxidase family of extracellular proteins includes LOX and four LOX-like enzymes, which are responsible for the deamination of peptidyl lysine residues of collagens and elastin. They also catalyze inter- and intra-crosslinking reactions. Overexpression of LOX may cause severe fibrotic degeneration due to its high resistance to degradative enzymes. Procollagen C-proteinase activity processes LOX from a precursor protein to a mature form. Activation of LOX occurs in normal developing and adult skin, and alterations in LOX expression and activity are associated with skin aging and senescence. LOX is crucial for development of the cardiovascular and respiratory systems. In addition, LOX plays a role in cancer, wound healing, as well as cell motility, chemotaxis and differentiation.
Product Categories/Family for anti-LOX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
46,944 Da
NCBI Official Full Name
lysyl oxidase
NCBI Official Synonym Full Names
lysyl oxidase
NCBI Official Symbol
LOX
NCBI Protein Information
protein-lysine 6-oxidase
UniProt Protein Name
Protein-lysine 6-oxidase
UniProt Gene Name
LOX
UniProt Entry Name
LYOX_HUMAN
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Product Notes
The LOX lox (Catalog #AAA30405) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LOX Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's LOX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:100 ICC: 1:100-1:500 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the LOX lox for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LOX, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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