IHC (Immunohistochemistry)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil using AAA14660 and following by peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.)
Mouse anti-Human Mast Cell Chymase Monoclonal Antibody | anti-CMA1 antibody
Mast Cell Chymase (BSA & Azide Free)
Gene Names
CMA1; CYH; MCT1; chymase; MGC119890; MGC119891
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Protein G affinity chromatography.
Synonyms
Mast Cell Chymase, Antibody; Mast Cell Chymase (BSA & Azide Free); Anti -Mast Cell Chymase (BSA & Azide Free); anti-CMA1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F258
Specificity
Recognizes human Mast Cell Chymase. Binding of this antibody to chymase is not reduced in the presence of heparin, suggesting that the antibody does not bind to the heparin-binding domain of chymase.
Purity/Purification
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide.
Concentration
1mg/ml (varies by lot)
Sequence
Not determined
Applicable Applications for anti-CMA1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:2000 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher.
Grade
Affinity Purified
Immunogen
Purified human skin chymase.
Cellular Localization
Cytoplasmic
Positive Control
Tonsil
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
IHC (Immunohistochemistry)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil using AAA14660 and following by peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.)
IHC (Immunohistochemistry)
(Immunohistochemistry of formalin-fixed, paraffin-embedded human tonsil using AAA14660 and following by peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of mast cells.)
Related Product Information for anti-CMA1 antibody
Mast cells contain a number of preformed chemical mediators such as histamine, chymase, carboxypeptidase and proteolytic tryptase. A substantial quantity of chymase is estimated to be found in mast cells of skin and lung and suggestes this enzyme plays a major role in mast cell mediated events. Human mast cell chymase is considered to be an important marker of mast cells as well as an important mediator of inflammation.
Product Categories/Family for anti-CMA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
mast cell chymase
NCBI Official Synonym Full Names
chymase 1, mast cell
NCBI Official Symbol
CMA1
NCBI Official Synonym Symbols
CYH; MCT1; chymase; MGC119890; MGC119891
NCBI Protein Information
chymase; alpha-chymase; chymase, heart; OTTHUMP00000178751; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I
UniProt Protein Name
Chymase
UniProt Gene Name
CMA1
UniProt Synonym Gene Names
CYH; CYM
UniProt Entry Name
CMA1_HUMAN
Similar Products
Product Notes
The CMA1 cma1 (Catalog #AAA14660) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mast Cell Chymase (BSA & Azide Free) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mast Cell Chymase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Recommended Dilutions:Immunohistochemistry (Formalin/paraffin): 1:2000 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CMA1 cma1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Not determined. It is sometimes possible for the material contained within the vial of "Mast Cell Chymase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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