IF (Immunofluorescence)
(Figure 3 and 4: ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AAA11724 (1;100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucelus (blue).Figure 5 and 6: ICC/IF analysis of MIF in HeLa cells line. The cell was stained with AAA11724 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue))
Mouse anti-Human MIF Monoclonal Antibody | anti-MIF antibody
MIF antibody
Gene Names
MIF; GIF; GLIF; MMIF
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
MIF, Antibody; MIF antibody; Macrophage migration inhibitory factor; GLIF; MMIF; MIF; EC 5.3.2.1; Phenylpyruvate tautomerase; Glycosylation-inhibiting factor; GIF; macrophage migration inhibitory factor (glycosylation-inhibiting factor),; anti-MIF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
4E4
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid in phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (determined by BCA assay) (varies by lot)
Sequence Length
115
Applicable Applications for anti-MIF antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
The antibody has been tested by ELISA and Western Blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Immunogen
Recombinant human MIF (1-115 aa) purified from E. coli
Preparation and Storage
Can be stored +2 to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(Figure 3 and 4: ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AAA11724 (1;100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucelus (blue).Figure 5 and 6: ICC/IF analysis of MIF in HeLa cells line. The cell was stained with AAA11724 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue))
IF (Immunofluorescence)
(Figure 3 and 4: ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AAA11724 (1;100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucelus (blue).Figure 5 and 6: ICC/IF analysis of MIF in HeLa cells line. The cell was stained with AAA11724 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue))
WB (Western Blot)
(Figure 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysateFigure 2: The cell lysates of HL-60 was resolved by SDS-PAGE, transfered to PVDF membrane and probed with anit-human MIF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.)
WB (Western Blot)
(Figure 1: The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysateFigure 2: The cell lysates of HL-60 was resolved by SDS-PAGE, transfered to PVDF membrane and probed with anit-human MIF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and ECL detection system.)
Related Product Information for anti-MIF antibody
The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration.
Product Categories/Family for anti-MIF antibody
References
Weiser WY, et al., (1989) Proc Natl Acad Sci. 86:7522-7526.; Bernhagen J, et al., (1994) Biochemistry. 33:14144-14155; Richard B, et al., (1996) FASEB J. 10:1607-1613
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
macrophage migration inhibitory factor
NCBI Official Synonym Full Names
macrophage migration inhibitory factor
NCBI Official Symbol
MIF
NCBI Official Synonym Symbols
GIF; GLIF; MMIF
NCBI Protein Information
macrophage migration inhibitory factor
UniProt Protein Name
Macrophage migration inhibitory factor
UniProt Gene Name
MIF
UniProt Synonym Gene Names
GLIF; MMIF; MIF; GIF
Similar Products
Product Notes
The MIF mif (Catalog #AAA11724) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MIF antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA and Western Blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the MIF mif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.