Rabbit anti-Human MonoMethyl-Histone H3-K18 Monoclonal Antibody | anti-H3 antibody

MonoMethyl-Histone H3-K18 Rabbit mAb

Cat. Num. AAA28631

• Reactivity: Human
• Applications: Western Blot, Dot Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, ELISA, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Affinity purification
• Synonyms: MonoMethyl-Histone H3-K18, Antibody; MonoMethyl-Histone H3-K18 Rabbit mAb; H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; MonoMethyl-Histone H3-K18; anti-H3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
• Sequence: APRKQ

• Applicable Applications for anti-H3 antibody: Western Blot (WB), Dot Blot (DB), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), ELISA (EIA), Chromatin Immunoprecipitation (ChIP), CUT&Tag
• Application Notes: WB: 1:500-1:1000; DB: 1:500-1:1000; IHC-P: 1:50-1:200; IF/ICC: 1:50-1:200; IP: 0.5ug-4ug antibody for 200ug-400ug extracts of whole cells; ELISA: Recommended starting concentration is 1ug/mL.; Please optimize the concentration based on your specific assay requirements.; ChIP: 5ug antibody for 5ug-10ug of Chromatin; CUT&Tag: 10? cells /1 ug
• Cross Reactivity: Human, Mouse, Rat, Other (Wide Range Predicted)
• Immunogen: A synthetic monomethylated peptide around K18 of human histone H3 (P68431).
• Modification: MonoMethyl K9
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

ChIP (Chromatin Immunoprecipitation)

(Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody (AAA28631) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)

IP (Immunoprecipitation)

(Immunoprecipitation analysis of 600 ug extracts of 293F cells using 5 ug MonoMethyl-Histone H3-K18 antibody (AAA28631). Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K18 antibody (AAA28631) at a dilution of 1:1000.)

IF (Immunofluorescence)

(Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K18 Rabbit mAb (AAA28631) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K18 Rabbit mAb (AAA28631) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry)

(Immunohistochemistry analysis of paraffin-embedded Rat brain using MonoMethyl-Histone H3-K18 Rabbit mAb (AAA28631) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Mouse lung using MonoMethyl-Histone H3-K18 Rabbit mAb (AAA28631) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MonoMethyl-Histone H3-K18 Rabbit mAb (AAA28631) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

DB (Dot Blot)

(Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K18 antibody (AAA28631) at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of various lysates using MonoMethyl-Histone H3-K18 Rabbit mAb (AAA28631) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

Application Data

(CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina(RK20265) from 10? K562 cells with 1ug MonoMethyl-Histone H3-K18 Rabbit mAb(AAA28631), along with a Goat Anti-Rabbit IgG(H+L). The CUT&Tag results indicate the enrichment pattern of H3K18Me1 in representative gene loci (MYT1), as shown in figure.)

Related Product Information for anti-H3 antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Product Categories/Family for anti-H3 antibody

Epigenetics Nuclear Signaling; Protein phosphorylation; Signal Transduction; MAPK-Erk Signaling Pathway; Methylated Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4504299
• NCBI GeneID: 8290/8350
• NCBI Accession #: NP_003484.1
• NCBI GenBank Nucleotide #: NM_003493.2
• UniProt Accession #: Q16695; P68431
• Molecular Weight: Calculated MW: 16kDa; Observed MW: 17kDa
• UniProt Protein Name: Histone H3.1t
• UniProt Gene Name: HIST3H3
• UniProt Synonym Gene Names: H3FT; H3/t; H3t
• UniProt Entry Name: H31T_HUMAN