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FCM (Flow Cytometry)
(Flow cytometric analysis of 293Tcells with NDUFB8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit NDUFB8 Monoclonal Antibody | anti-NDUFB8 antibody
NDUFB8 Antibody
Gene Names
NDUFB8; ASHI; CI-ASHI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunocytochemistry
Purity
ProA affinity purified
Synonyms
NDUFB8; Monoclonal Antibody; NDUFB8 Antibody; ASHI antibody; CI-ASHI antibody; Complex I ASHI subunit antibody; Complex I-ASHI antibody; mitochondrial antibody; NADH dehydrogenase (ubiquinone) 1 beta subcomplex 8 19kDa antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 mitochondrial antibody; NADH-ubiquinone oxidoreductase ASHI subunit antibody; NDUB8_HUMAN antibody; NDUFB8 antibody; anti-NDUFB8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JG61-36
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-NDUFB8 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50
IP: 1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50
IP: 1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein within human NDUFB8 aa 30-130.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of 293Tcells with NDUFB8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of 293Tcells with NDUFB8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-NDUFB8 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of NDUFB8 on different lysates using anti-NDUFB8 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: A549 Lane 3: Mouse heart Lane 4: Rat spleen)
WB (Western Blot)
(Western blot analysis of NDUFB8 on different lysates using anti-NDUFB8 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: A549 Lane 3: Mouse heart Lane 4: Rat spleen)
Related Product Information for anti-NDUFB8 antibody
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Product Categories/Family for anti-NDUFB8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
186
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa
NCBI Official Symbol
NDUFB8
NCBI Official Synonym Symbols
ASHI; CI-ASHI
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial; complex I-ASHI; complex I ASHI subunit; NADH-ubiquinone oxidoreductase ASHI subunit; NADH:ubiquinone ox
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial
UniProt Gene Name
NDUFB8
UniProt Synonym Gene Names
CI-ASHI
UniProt Entry Name
NDUB8_HUMAN
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Product Notes
The NDUFB8 ndufb8 (Catalog #AAA30488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFB8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50 IP: 1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the NDUFB8 ndufb8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB8, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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