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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Neuropilin-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit Neuropilin-1 Monoclonal Antibody | anti-NRP1 antibody
Neuropilin-1 Antibody
Gene Names
NRP1; NP1; NRP; BDCA4; CD304; VEGF165R
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
Neuropilin-1; Monoclonal Antibody; Neuropilin-1 Antibody; A5 protein antibody; BDCA4 antibody; BLOOD DENDRITIC CELL ANTIGEN 4 antibody; CD304 antibody; Neuropilin-1 antibody; Neuropilin1 antibody; NP1 antibody; NPN1 antibody; NRP 1 antibody; NRP antibody; NRP1 antibody; NRP1_HUMAN antibody; transmembrane receptor antibody; Vascular endothelial cell growth factor 165 receptor antibody; VEGF165R antibody; anti-NRP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
ST05-30
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-NRP1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Neuropilin-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Neuropilin-1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining Neuropilin-1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Neuropilin-1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Neuropilin-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Neuropilin-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Neuropilin-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Neuropilin-1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Neuropilin-1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Neuropilin-1 on different lysates using anti-Neuropilin-1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: Mouse kidney Lane 4: Human liver)
WB (Western Blot)
(Western blot analysis of Neuropilin-1 on different lysates using anti-Neuropilin-1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Mouse liver Lane 3: Mouse kidney Lane 4: Human liver)
Related Product Information for anti-NRP1 antibody
Neuropilin is a type I transmembrane receptor that has been implicated in aspects of axon growth and guidance and has been shown to act as a high affinity receptor for class III semaphorins and vascular endothelial growth factor (VEGF). A closely related protein, neuropilin-2, shares a common domain structure and signifcant homology with neuropilin and also acts as a receptor for the class III semaphorins and VEGF. Both neuropilins are involved in regulating many physiological pathways including axonal guidance and angiogenesis, however they exhibit differential expression in the adult vasculature. Neuropilin-2 is polysialylated and expressed on the surface of dendritic cells. It is also expressed by venous and lymphatic endothelium. Neuropilin is expressed predominantly by arterial endothelium.
Product Categories/Family for anti-NRP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
103,134 Da
NCBI Official Full Name
neuropilin-1 isoform a
NCBI Official Synonym Full Names
neuropilin 1
NCBI Official Symbol
NRP1
NCBI Official Synonym Symbols
NP1; NRP; BDCA4; CD304; VEGF165R
NCBI Protein Information
neuropilin-1; transmembrane receptor; vascular endothelial cell growth factor 165 receptor
UniProt Protein Name
Neuropilin-1
UniProt Gene Name
NRP1
UniProt Synonym Gene Names
NRP; VEGF165R
UniProt Entry Name
NRP1_HUMAN
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Product Notes
The NRP1 nrp1 (Catalog #AAA30107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neuropilin-1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neuropilin-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the NRP1 nrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuropilin-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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