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Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Oct-2 Mouse Monoclonal Antibody (OCT2/2137) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Mouse OCT-2 (POU2F2) Monoclonal Antibody | anti-OCT-2 antibody
OCT-2 (POU2F2) (B-Cell Marker)
Gene Names
POU2F2; OCT2; OTF2; Oct-2
Reactivity
Human. Others not known.
Applications
ELISA, Western Blot, Immunohistochemistry
Synonyms
OCT-2 (POU2F2), Antibody; OCT-2 (POU2F2) (B-Cell Marker); Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; POU domain class 2 transcription factor 2; POU2F2; anti-OCT-2 antibody
Host
Mouse
Reactivity
Human. Others not known.
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
OCT2/2137
Specificity
Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. Oct-2 expression can be used as a marker of B-cell lineage and differentiation. Germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells show high level expression of Oct-2. Additionally, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, Hodgkin lymphoma display increased expression of Oct-2. Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines have high levels of expression. In spite of scanty evidence for Oct-2 expression in T cells, it is believed that this factor participates in transcriptional regulation during T-cell activation.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
479
Applicable Applications for anti-OCT-2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes
ELISA: For coating, order Ab without BSA.
WB: 1-2ug/ml
IHC-F: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
WB: 1-2ug/ml
IHC-F: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Immunogen
Recombinant fragment of human OCT2 protein (around aa 112-297)
Cellular Localization
Nuclear
Hu-chromosome Location
19q13.2
Positive Control
Raji or U-937 cells. Spleen or Tonsil.
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Oct-2 Mouse Monoclonal Antibody (OCT2/2137) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Application Data
(Analysis of Protein Array containing more than 19, 000 full-length human proteins using Oct-2 Mouse Monoclonal Antibody (OCT2/2137) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE
(SDS-PAGE Analysis Purified Oct-2 Mouse Monoclonal Antibody (OCT2/2137). Confirmation of Integrity and Purity of Antibody.)
SDS-PAGE
(SDS-PAGE Analysis Purified Oct-2 Mouse Monoclonal Antibody (OCT2/2137). Confirmation of Integrity and Purity of Antibody.)
WB (Western Blot)
(Western Blot Analysis (A) Recombinant Protein (B) Daudi Cell lysate Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
WB (Western Blot)
(Western Blot Analysis (A) Recombinant Protein (B) Daudi Cell lysate Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
WB (Western Blot)
(Western Blot Analysis Ramos Cell lysate using Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
WB (Western Blot)
(Western Blot Analysis Ramos Cell lysate using Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Tonsil stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human Lymph Node stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).)
References
Gibson SE, et al. Am J Clin Pathol. 2006; 126:916-24. Browne P, et al. Am J Clin Pathol. 2003; 120:767-77.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60kDa
NCBI Official Full Name
POU domain, class 2, transcription factor 2 isoform 1
NCBI Official Synonym Full Names
POU class 2 homeobox 2
NCBI Official Symbol
POU2F2
NCBI Official Synonym Symbols
OCT2; OTF2; Oct-2
NCBI Protein Information
POU domain, class 2, transcription factor 2
UniProt Protein Name
POU domain, class 2, transcription factor 2
UniProt Gene Name
POU2F2
UniProt Synonym Gene Names
OCT2; OTF2; Oct-2; OTF-2
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Product Notes
The OCT-2 pou2f2 (Catalog #AAA23902) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The OCT-2 (POU2F2) (B-Cell Marker) reacts with Human. Others not known. and may cross-react with other species as described in the data sheet. AAA Biotech's OCT-2 (POU2F2) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Formalin. ELISA: For coating, order Ab without BSA. WB: 1-2ug/ml IHC-F: 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the OCT-2 pou2f2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OCT-2 (POU2F2), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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