Rabbit Osteopontin Monoclonal Antibody | anti-Spp1 antibody

Recombinant Anti-Osteopontin Antibody, Rabbit Monoclonal

Cat. Num. AAA27731

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1; MGC110940
• Applications: ELISA, Immunohistochemistry, Flow Cytometry
• Synonyms: Osteopontin, Antibody; Recombinant Anti-Osteopontin Antibody, Rabbit Monoclonal; Osteopontin/SPP1/ETA-1 Antibody, Rabbit MAb; secreted phosphoprotein 1; Anti-BNSP Antibody; Anti-BSPI Antibody; Anti-ETA-1 Antibody; Anti-OPN Antibody; anti-Spp1 antibody

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Human Osteopontin/SPP1/ETA-1
• Form/Format: 0.2um filtered solution in PBS

• Applicable Applications for anti-Spp1 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC)
• Application Notes: IHC-P: 1:1000-1:4000; FC: 1:25-1:100; ELISA: 1:5000-1:10000; This antibody can be used at 1:5000-1:10000 with the appropriate secondary reagents to detect Human SPP1.; Optimal concentrations/dilutions should be determined by the end user.
• Immunogen: Recombinant Human Osteopontin/SPP1/ETA-1 protein
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Osteopontin/SPP1/ETA-1 (rh Osteopontin/SPP1/ETA-1).
• Preparation and Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohistochemistry)

(Immunochemical staining of human SPP1 in human hepatoma with rabbit monoclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry)

(Immunochemical staining of human SPP1 in human kidney with rabbit monoclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

FCM (Flow Cytometry)

(Flow cytometric analysis of purified anti-human Spp1 on U937 cells. U937 cells were treated according to manufacturer's manual (BD Pharmingen'), and stained with Purified Rabbit anti-Spp1 (Bold line hisgram), To demonstrate specificity of staining the binding of anti-human Spp1 (10352-R001) was blocked by the preincubation of the purified antibody with molar excess of recombinant human Spp1 (5 ug,) for 1 hour (dashed line hisgram), then stained with a FITC-conjugated second step antibody.)

Related Product Information for anti-Spp1 antibody

Osteopontin, also known as Secreted phosphoprotein 1, Bone sialoprotein 1, BSP-1, OPN, and SPP1, is a member of the osteopontin family and a SIBLING glycoprotein. Osteopontin has been classified as T-helper 1 cytokine and thus believed to exacerbate inflammation in several chronic inflammatory diseases, including atherosclerosis. Besides proinflammatory functions, physiologically Osteopontin is a potent inhibitor of mineralization, it prevents ectopic calcium deposits and is a potent inducible inhibitor of vascular calcification. Osteopontin is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Osteopontin recruitment functions of inflammatory cells are thought to be mediated through its adhesive domains, especially the arginine-glycine-aspartate (RGD) sequence that interacts with several integrin heterodimers. Osteopontin has emerged as a potential biomarker and mediator in cardiovascular disease. In the context of atherosclerosis, OPN is generally regarded as a proinflammatory and proatherogenic molecule. However, the role of OPN in vascular calcification (VC), which is closely related to chronic and active inflammation, is that of a negative regulator because it is an inhibitor of calcification and an active inducer of decalcification. Extensive research has demonstrated the pivotal participation of Osteopontin in the regulation of cell signaling which controls neoplastic and malignant transformation. The elevated expression of Osteopontin has been observed in a variety of cancers. It has been linked with tumor metastasis and signifies a poor prognosis for the patient.

References

Scatena M, et al. (2007) Osteopontin: a multifunctional molecule regulating chronic inflammation and vascular disease. Arterioscler Thromb Vasc Biol. 27(11): 2302-9.Johnston NI, et al. (2008) Osteopontin as a target for cancer therapy. Front Biosci. 13: 4361-72.Cho HJ, et al. (2009) Osteopontin: a multifunctional protein at the crossroads of inflammation, atherosclerosis, and vascular calcification. Curr Atheroscler Rep. 11(3): 206-13.Waller AH, et al. (2010) Osteopontin in cardiovascular disease: a potential therapeutic target. Cardiol Rev. 18(3): 125-31.Shevde LA, et al. (2010) Osteopontin: an effector and an effect of tumor metastasis. Curr Mol Med. 10(1): 71-81.

NCBI and Uniprot Product Information

• NCBI GI #: 91206462
• NCBI GeneID: 6696
• NCBI Accession #: NP_001035147.1
• NCBI GenBank Nucleotide #: NM_001040058.1
• UniProt Accession #: P10451; Q15681; Q15682; Q15683; Q8NBK2; Q96IZ1; Q3LGB0; Q4W597; Q567T5
• NCBI Official Full Name: osteopontin isoform a
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1; MGC110940
• NCBI Protein Information: osteopontin; SPP-1; uropontin; nephropontin; osteopontin-C; OTTHUMP00000161173; OTTHUMP00000161174; OTTHUMP00000218555; OTTHUMP00000218556; SPP1/CALPHA1 fusion; bone sialoprotein 1; bone sialoprotein I; urinary stone protein; early T-lymphocyte activation
• UniProt Protein Name: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1
• UniProt Entry Name: OSTP_HUMAN