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FCM (Flow Cytometry)
(Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with AAA11732 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black))
Mouse anti-Human PARP2 Monoclonal Antibody | anti-PARP2 antibody
PARP2 antibody
Gene Names
PARP2; ARTD2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-A affinity chromatography
Synonyms
PARP2, Antibody; PARP2 antibody; Poly (ADP-ribose) polymerase 2; ADPRT2; ADPRTL2; ADPRTL3; ARTD2; pADPRT-2; PARP-2; anti-PARP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
AT29G4
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
583
Applicable Applications for anti-PARP2 antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
Recombinant human PARP2 (233-583aa) purified from E. coli
Usage
The antibody has been tested by ELISA, Western Blot, ICC/IF and FC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Preparation and Storage
Can be stored at +2°C to +8°C for one week.
For long term storage , aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage , aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
FCM (Flow Cytometry)
(Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with AAA11732 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black))
FCM (Flow Cytometry)
(Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with AAA11732 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black))
IF (Immunofluorescence)
(ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with AAA11732 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with AAA11732 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Raji cell lysate Lane 2. : NIH-3T3 cell lysate)
WB (Western Blot)
(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Raji cell lysate Lane 2. : NIH-3T3 cell lysate)
WB (Western Blot)
(The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system)
WB (Western Blot)
(The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system)
Related Product Information for anti-PARP2 antibody
PARP2 is poly (ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly (ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
Product Categories/Family for anti-PARP2 antibody
References
Ame JC, Rolli V, Schreiber V et al. (1999). J. Biol. Chem. 274 (25): 17860-8.; Schreiber V, Ame JC, Dolle P et al. (2002). J. Biol. Chem. 277 (25): 23028-36.; Maeda Y, Hunter TC, Loudy DE et al. (2006). J. Biol. Chem. 281 (14): 9600-6.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly(ADP-ribose) polymerase 2
NCBI Official Symbol
PARP2
NCBI Official Synonym Symbols
ARTD2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2
NCBI Protein Information
poly [ADP-ribose] polymerase 2
UniProt Protein Name
Poly [ADP-ribose] polymerase 2
UniProt Gene Name
PARP2
UniProt Synonym Gene Names
ADPRT2; ADPRTL2; PARP-2; hPARP-2; ARTD2; ADPRT-2; pADPRT-2
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Product Notes
The PARP2 parp2 (Catalog #AAA11732) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PARP2 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PARP2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the PARP2 parp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PARP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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