<
Mouse anti-Human PDL2 Monoclonal Antibody | anti-PDL2 antibody
PDL2 Antibody [7C1]
Gene Names
PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Protein A purified
Synonyms
PDL2, Antibody; PDL2 Antibody [7C1]; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2; Programmed cell death 1 ligand 2; Butyrophilin B7-DC; PD-1 ligand 2; programmed cell death 1 ligand; anti-PDL2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
7C1
Purity/Purification
Protein A purified
Form/Format
Liquid
Supplied in PBS containing 0.02% sodium azide.
Supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
273
Applicable Applications for anti-PDL2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB at 0.5-1 ug/mL
IHC starting at 2 ug/mL
IF start at 20 ug/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
IHC starting at 2 ug/mL
IF start at 20 ug/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
Immunogen
PD-L2 antibody was raised against the extracellular domain of human PD-L2.
Conjugate
Unconjugated
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
As with all antibodies care should be taken to avoid repeated freeze thaw cycles.
Antibodies should not be exposed to prolonged high temperatures.
FCM (Flow Cytometry)
(Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.)
IHC (Immunohistchemistry)
(Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [7C1]Blue: DAPI staining)
IF (Immunofluorescence)
(Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [7C1]Blue: DAPI staining)
ICC (Immunocytochemistry)
(Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)
WB (Western Blot)
(Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml)
Related Product Information for anti-PDL2 antibody
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by co gnate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PD-L1 and PD-L2, both of which are thought act as a negative regulator of T cell activation. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through and alternative receptor from PD-1.
Product Categories/Family for anti-PDL2 antibody
References
Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90. Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95. LaGier J and Pober JS. Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1). Hum. Immunol. 2006; 67:568-78. Zhang Y, Chung Y, Bishop C, et al. Regulation of T cell activation and tolerance by PDL2. Proc. Natl. Acad. Sci. USA 2006; 103:11695-700.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 30 kDa
Observed: 38 kDa
Observed: 38 kDa
NCBI Official Full Name
programmed cell death 1 ligand 2
NCBI Official Synonym Full Names
programmed cell death 1 ligand 2
NCBI Official Symbol
PDCD1LG2
NCBI Official Synonym Symbols
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
NCBI Protein Information
programmed cell death 1 ligand 2
UniProt Protein Name
Programmed cell death 1 ligand 2
UniProt Gene Name
PDCD1LG2
UniProt Synonym Gene Names
B7DC; CD273; PDCD1L2; PDL2; PD-1 ligand 2; PD-L2; PDCD1 ligand 2; Programmed death ligand 2; B7-DC
