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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit PHD1/Prolyl Hydroxylase Monoclonal Antibody | anti-PHD1 antibody
PHD1/Prolyl Hydroxylase Antibody
Gene Names
EGLN2; EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
ProA affinity purified
Synonyms
PHD1/Prolyl Hydroxylase; Monoclonal Antibody; PHD1/Prolyl Hydroxylase Antibody; DKFZp434E026 antibody; EGL nine (C.elegans) homolog 2 antibody; Egl nine homolog 2 (C. elegans) antibody; Egl nine homolog 2 antibody; EGLN 2 antibody; EGLN2 antibody; EGLN2_HUMAN antibody; EIT 6 antibody; EIT6 antibody; Estrogen-induced tag 6 antibody; HIF P4H 1 antibody; HIF PH1 antibody; HIF prolyl hydroxylase 1 antibody; HIF-PH1 antibody; HIF-prolyl hydroxylase 1 antibody; HIFPH 1 antibody; HIFPH1 antibody; HPH 3 antibody; HPH-1 antibody; HPH-3 antibody; HPH3 antibody; Hypoxia inducible factor prolyl hydroxylase 1 antibody; Hypoxia-inducible factor prolyl hydroxylase 1 antibody; P4H1 antibody; PHD 1 antibody; PhD1 antibody; prolyl hydroxylase domain containing protein 1 antibody; Prolyl hydroxylase domain-containing protein 1 antibody; anti-PHD1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
SP00-48
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PHD1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-5000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with PHD1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHD1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHD1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHD1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHD1 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC12 Lane 3: NIH/3T3)
WB (Western Blot)
(Western blot analysis of PHD1 on different lysates using anti-PHD1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC12 Lane 3: NIH/3T3)
Related Product Information for anti-PHD1 antibody
Prolyl hydroxylase domain proteins HIF PHD1, HIF PHD2 and HIF PHD3 (known as PHD1, PHD2 and PHD3 in rodents, respectively) can hydroxylate HIF-alpha subunits. Hypoxia-inducible factor (HIF) is a transcriptional regulator important in several aspects of oxygen homeostasis. The prolyl hydroxylases catalyze the posttranslational formation of 4-hydroxyproline in HIF-alpha proteins. HIF PHD1, which is widely expressed, with highest levels of expression in testis, functions as a cellular oxygen sensor and is important in cell growth regulation. HIF PHD1 can localize to the nucleus or the cytoplasm and is also detected in hormone responsive tissues, such as normal and cancerous mammary, ovarian and prostate epithelium. HIF PHD1 is encoded by EGLN2, which maps to chromosome 19q13.3. HIF PHD2 is regarded as the main cellular oxygen sensor, as RNA interference against HIF PHD2, but not HIF PHD1 or HIF PHD3, is enough to stabilize HIF-1alpha in normoxia. HIF PHD2, a direct HIF target gene, is expressed mainly in skeletal muscle, heart, kidney and brain. HIF PHD3 may play a role in the regulation of cell growth in muscle cells and in apoptosis in neuronal tissue. HIF PHD3 is widely expressed, although the highest levels can be detected in placenta and he
Product Categories/Family for anti-PHD1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
407
NCBI Official Full Name
Egl nine homolog 2
NCBI Official Synonym Full Names
egl-9 family hypoxia-inducible factor 2
NCBI Official Symbol
EGLN2
NCBI Official Synonym Symbols
EIT6; PHD1; HPH-1; HPH-3; HIFPH1; HIF-PH1
NCBI Protein Information
egl nine homolog 2; estrogen-induced tag 6; HIF-prolyl hydroxylase 1; hypoxia-inducible factor prolyl hydroxylase 1; prolyl hydroxylase domain-containing protein 1
UniProt Protein Name
Egl nine homolog 2
UniProt Gene Name
EGLN2
UniProt Synonym Gene Names
EIT6; HIF-PH1; HIF-prolyl hydroxylase 1; HPH-1; PHD1
UniProt Entry Name
EGLN2_HUMAN
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Product Notes
The PHD1 egln2 (Catalog #AAA30022) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHD1/Prolyl Hydroxylase Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PHD1/Prolyl Hydroxylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-5000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PHD1 egln2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHD1/Prolyl Hydroxylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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