WB (Western Blot)
(Western Blot analysis of bacterial alkaline phosphatase tagged, using AAA14702.)
Mouse anti-Human Phosphatase, Alkaline, Bone Monoclonal Antibody
Mouse anti-Human Phosphatase, Alkaline, Human, Bone (BAP)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Synonyms
Phosphatase, Alkaline, Bone, Antibody; Mouse anti-Human Phosphatase, Alkaline, Human, Bone (BAP); Anti -Phosphatase, Alkaline, Human, Bone (BAP); anti-Phosphatase, Alkaline, Bone antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
0.G.2
Specificity
Recognizes Alkaline Phosphatase from human bone. Crossreactivity: Alkaline phosphatase from human placenta, calf intestine and bacterial.
Purity/Purification
Purified by Protein A affinity chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Phosphatase, Alkaline, Bone antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Other applications not tested. Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
Please inquire: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Please inquire: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Recommended Secondary Antibodies:
Please inquire: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Please inquire: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
Immunogen
Purified alkaline phosphatase (native) from human bone.
Grade
Affinity Purified
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
WB (Western Blot)
(Western Blot analysis of bacterial alkaline phosphatase tagged, using AAA14702.)
WB (Western Blot)
(Western Blot analysis of bacterial alkaline phosphatase tagged, using AAA14702.)
Related Product Information for anti-Phosphatase, Alkaline, Bone antibody
Alkaline phosphatase (AP) is a ubiquitously expressed enzyme which removes phosphate groups from target molecules, including DNA, RNA and alkaloids, under alkaline conditions, and is present at higher concentrations in the placenta (placental AP), intestines (intestinal AP) and liver/bone/kidney (tissue non-specific AP). Although the exact biochemical function of BAP is uncertain, measurement of the serum levels of BAP can be used as a biochemical indicator of bone turnover. Conditions which present with a decrease in the level of BAP (hypophosphatasemia) include the inherited bone-deforming disorder hypophosphatasia and osteoporosis, while an increase in BAP (hyperphosphatasemia) is associated with Paget's disease, bone fractures and osteosarcomas.
Product Categories/Family for anti-Phosphatase, Alkaline, Bone antibody
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The Phosphatase, Alkaline, Bone (Catalog #AAA14702) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Human Phosphatase, Alkaline, Human, Bone (BAP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphatase, Alkaline, Bone can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Other applications not tested. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: Please inquire: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Please inquire: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo. Researchers should empirically determine the suitability of the Phosphatase, Alkaline, Bone for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphatase, Alkaline, Bone, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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