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FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Rabbit RbAP48 Monoclonal Antibody | anti-RbAP48 antibody
RbAP48 Antibody
Gene Names
RBBP4; NURF55; RBAP48; lin-53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Purity
ProA affinity purified
Synonyms
RbAP48; Monoclonal Antibody; RbAP48 Antibody; CAF I p48 antibody; CAF-1 subunit C antibody; CAF-I 48 kDa subunit antibody; CAF-I p48 antibody; Chromatin assembly factor 1 subunit C antibody; Chromatin assembly factor I p48 subunit antibody; Chromatin assembly factor/CAF 1 p48 subunit antibody; Histone-binding protein RBBP4 antibody; MSI1 protein homolog antibody; Nucleosome-remodeling factor subunit RBAP48 antibody; NURF55 antibody; RB binding protein 4 chromatin remodeling factor antibody; RbAp 48 antibody; RBAP48 antibody; RBBP-4 antibody; RBBP4 antibody; RBBP4_HUMAN antibody; Retinoblastoma binding protein 4 antibody; Retinoblastoma binding protein p48 antibody; Retinoblastoma-binding protein 4 antibody; Retinoblastoma-binding protein p48 antibody; anti-RbAP48 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Clone Number
JU37-47
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-RbAP48 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
IP:1:10-1:50
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
IP:1:10-1:50
FC/FACS: 1:50-1:100
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with RbAP48 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RbAP48 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RbAP48 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining RbAP48 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RbAP48 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution. Positive control: Lane 1: NIH-3T3 Lane 2: Hela Lane 3: Rat brain Lane 4: Mouse testis)
WB (Western Blot)
(Western blot analysis of RbAP48 on different lysates using anti-RbAP48 antibody at 1/500 dilution. Positive control: Lane 1: NIH-3T3 Lane 2: Hela Lane 3: Rat brain Lane 4: Mouse testis)
Related Product Information for anti-RbAP48 antibody
In the intact cell, DNA is closely associated with histones and other nuclear proteins to form chromatin.The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation, and an increased accessiblity of DNA to transcription factors. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases. The retinoblastoma binding proteins RbAp46 and RbAp48 have been identified as histone binding proteins, and they are components of the histone deacetylase complex.
Product Categories/Family for anti-RbAP48 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
425
NCBI Official Full Name
Histone-binding protein RBBP4
NCBI Official Synonym Full Names
retinoblastoma binding protein 4
NCBI Official Symbol
RBBP4
NCBI Official Synonym Symbols
NURF55; RBAP48; lin-53
NCBI Protein Information
histone-binding protein RBBP4; RBBP-4; CAF-I p48; CAF-1 subunit C; CAF-I 48 kDa subunit; MSI1 protein homolog; retinoblastoma-binding protein 4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 sub
UniProt Protein Name
Histone-binding protein RBBP4
UniProt Gene Name
RBBP4
UniProt Synonym Gene Names
RBAP48; CAF-1 subunit C; CAF-I 48 kDa subunit; CAF-I p48; RBBP-4
UniProt Entry Name
RBBP4_HUMAN
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Product Notes
The RbAP48 rbbp4 (Catalog #AAA30415) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RbAP48 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RbAP48 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 IP:1:10-1:50 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the RbAP48 rbbp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RbAP48, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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