Rabbit anti-Human, Mouse RHEB Monoclonal Antibody | anti-RHEB antibody

RHEB Antibody

Cat. Num. AAA30496

• Gene Names: RHEB; RHEB2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: RHEB; Monoclonal Antibody; RHEB Antibody; Ras homolog enriched in brain 2; formerly antibody; GTP binding protein Rheb antibody; GTP-binding protein Rheb antibody; MGC111559 antibody; Ras homolog enriched in brain 2 antibody; Ras homolog enriched in brain antibody; RHEB 2 antibody; Rheb antibody; RHEB_HUMAN antibody; RHEB2 antibody; RHEB2; anti-RHEB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Clone Number: JG37-12
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-RHEB antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF),Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ICC: 1:50-1:200; FC/FACS: 1:50-1:100
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein within human RHEB aa 1-250.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

FCM (Flow Cytometry)

(Flow cytometric analysis of A431 cells with RHEB antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry)

(ICC staining RHEB in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry)

(ICC staining RHEB in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-RHEB antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RHEB antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RHEB antibody. Counter stained with hematoxylin.)

WB (Western Blot)

(Western blot analysis of RHEB on mouse placenta tissue lysate using anti-RHEB antibody at 1/1, 000 dilution.)

Related Product Information for anti-RHEB antibody

H-, K- and N-Ras represent the prototype members of a family of small G proteins which are frequently activated to an oncogenic state in a wide variety of human tumors. Activation is due to point mutations at position 12 or 61 within their coding sequence. Such mutations cause these proteins to be constitutively converted to their active GTP-bound rather than the inactive GDP-bound state. The related human R-Ras gene was initially cloned by low stringency hybridization methods. Position 38 or 87 mutants of R-Ras (analogous to positions 12 and 61 in H-Ras) have been shown to be capable of activating oncogenic function. Ras p21 in its active GTP binding state binds to Raf-1, resulting in activation of the MAP kinase signaling cascade. An additional member of the Ras family, Rheb (Ras-related GTP-binding protein), also interacts with Raf-1. This interaction is potentiated by growth factors and agents that increase cAMP levels.

Product Categories/Family for anti-RHEB antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 6919957
• NCBI GeneID: 6009
• UniProt Accession #: Q15382; Q53Y56; Q99444; B3KWN6; D3DX13
• Molecular Weight: 184
• NCBI Official Full Name: GTP-binding protein Rheb
• NCBI Official Synonym Full Names: Ras homolog enriched in brain
• NCBI Official Symbol: RHEB
• NCBI Official Synonym Symbols: RHEB2
• NCBI Protein Information: GTP-binding protein Rheb; Ras homolog enriched in brain 2
• UniProt Protein Name: GTP-binding protein Rheb
• UniProt Gene Name: RHEB
• UniProt Synonym Gene Names: RHEB2
• UniProt Entry Name: RHEB_HUMAN