IF (Immunofluorescence)
(Immunofluorescence (IFC) analysis of PC3 cells using AAA14697 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse Ribonuclease inhibitor 1 Monoclonal Antibody | anti-RNH1 antibody
Ribonuclease inhibitor 1 (RNH1)
Gene Names
RNH1; RAI; RNH; MGC4569; MGC18200; MGC54054
Applications
Immunocytochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Ribonuclease inhibitor 1, Antibody; Ribonuclease inhibitor 1 (RNH1); Anti -Ribonuclease inhibitor 1 (RNH1); anti-RNH1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
10C309
Specificity
Recognizes human Ribonuclease inhibitor 1
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RNH1 antibody
Immunofluoresence/Immunocytochemistry (IF/ICC), ELISA and Western Blot (WB).
Application Notes
WB: 1:500
IF/ICC: 1:100
Optimal dilutions to be determined by the researcher. Other applications not tested.
IF/ICC: 1:100
Optimal dilutions to be determined by the researcher. Other applications not tested.
Immunogen
Recombinant protein corresponding to aa7-461 from human Ribonuclease inhibitor 1 expressed in E. coli.
Hybridoma
FO myeloma cells with spleen cells form Balb/c mice.
Preparation and Storage
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
IF (Immunofluorescence)
(Immunofluorescence (IFC) analysis of PC3 cells using AAA14697 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunofluorescence)
(Immunofluorescence (IFC) analysis of PC3 cells using AAA14697 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot)
(Western Blot analysis of cell lysates (40ug) were resolved by SDS-PAGE, trasferred to PVDF membrane using AAA14697 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPLane 1: Jurkat cell lysateLane 2: HepG2 cell lysate)
WB (Western Blot)
(Western Blot analysis of cell lysates (40ug) were resolved by SDS-PAGE, trasferred to PVDF membrane using AAA14697 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRPLane 1: Jurkat cell lysateLane 2: HepG2 cell lysate)
Related Product Information for anti-RNH1 antibody
Ribonuclease inhibitor 1(RNH1) the placental ribonuclease inhibitor, is an acid protein with a molecular weight of 50kD. RNH1 is a member of a family of proteinaceous cytoplasmic RNase inhibitors that are expressed in many tissues and bind to both intracellular and extracellular RNases in the cytosol. RNH1 is a role in abolishing the ribonucleolytic and angiogenic activities of angiogenin.
Product Categories/Family for anti-RNH1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
ribonuclease inhibitor
NCBI Official Synonym Full Names
ribonuclease/angiogenin inhibitor 1
NCBI Official Symbol
RNH1
NCBI Official Synonym Symbols
RAI; RNH; MGC4569; MGC18200; MGC54054
NCBI Protein Information
ribonuclease inhibitor; OTTHUMP00000147628; OTTHUMP00000229864; OTTHUMP00000229865; OTTHUMP00000229866; OTTHUMP00000229870; OTTHUMP00000229871; OTTHUMP00000229872; placental RNase inhibitor; Placental ribonuclease inhibitor
UniProt Protein Name
Ribonuclease inhibitor
UniProt Gene Name
RNH1
UniProt Synonym Gene Names
PRI; RNH
UniProt Entry Name
RINI_HUMAN
Similar Products
Product Notes
The RNH1 rnh1 (Catalog #AAA14697) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ribonuclease inhibitor 1 can be used in a range of immunoassay formats including, but not limited to, Immunofluoresence/Immunocytochemistry (IF/ICC), ELISA and Western Blot (WB). WB: 1:500 IF/ICC: 1:100 Optimal dilutions to be determined by the researcher. Other applications not tested. Researchers should empirically determine the suitability of the RNH1 rnh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ribonuclease inhibitor 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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